Looking at the chromosome location for a particular gene I find that the description varies depending on the source. Is there a gold standard?

More Harriet C T Groom's questions See All

How do I purify serum in unconventional way?

I plan to purify serum for serum-neutralization assay, by simply centrifuging the serum, collecting the supernatant, and discarding the debris. Has anyone ever done this before? If so, what speed...

05 February 2023 3,853 3 View

What is the best protocol for isolating neutrophils for investigating Mac-1 surface expression?

Hi, I have been isolating neutrophils for N-Formylmethionyl-leucyl-phenylalanine (FMLP) activation for a couple of months without success. In my protocol, I dilute heparinized whole blood with PBS...

15 May 2022 2,964 0 View

Is there any pilot study done on the validity of hamzat and dada questionnaire?

I'm looking for the validity and reliability of the questionnaire used in this study ' wheelchair accessibility to public buildings by people living with disabilitys' by hamzat and dada

25 February 2022 3,397 0 View

How does Programmatic Advertising relate to Geodemographics?

They are both within the sphere of contemporary digital marketing, each a different technique I guess, I'm just wondering what the direct links between them are? Do any programmatic advertising...

08 November 2021 9,220 1 View

Would you grant me permission to use your images of cubozoans?

I am working on a guide to invertebrates in the northern Gulf of Mexico for use on federal and state survey vessels to help improve identifications at sea. I am asking for permissions to use one...

01 September 2020 7,137 1 View

Is there a rule about the frequency conversion in photon, phonon, magnon conversions in crystal lattices?

We are trying to understand the role of basalt paramagnetism in soil to enhance plant growth. Apparently ELF waves interact with basalt in a way that is favorable for plant growth. The question is...

19 July 2020 9,108 0 View

Can i get help in understanding and interpretation of multiple alignment of a sequence after cloning & blasting?

I am working on genome & chromosomal properties of Bambara groundnut. My interest is in centromeric DNA -tandem repeats. I used primers from cowpea which is a closely related specie to bambara....

20 August 2019 7,454 3 View

What might cause the ~160bp peak in this library prepared using FFPE extracted RNA and Takara SMARTer kit?

I have prepared libraries for RNA-seq using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian. The input RNA was extracted from FFPE tissue. Some of the final libraries have...

16 June 2019 1,846 2 View

Which 16S universal primers can I use for bacterial identification that will target the V3 and V4 region?

16S rRNA profiling

22 February 2018 4,555 4 View

What type of statistical tool can I use for my research project?

My PICOT is as follows: for nurses at a jail setting, how does implementing a policy of EBP guidelines on TDM influence serum drug lab testing? i plan on comparing the percentage of completed...

15 March 2017 1,894 3 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

How many established theories of macroevolution?

There are seven (7) established theories of macroevolution viz. i) Chromosomal speciation theory, ii) Shifting balance theory, iii) Punctuated equilibrium theory, iv) Allopatric speciation theory,...

10 July 2024 9,679 1 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View

Why the secondary constriction region of chromosome i.e. known as NOR, is non-staining region?

Silver staining process is used to stain this region, why?

12 June 2024 4,517 1 View

How many chromosomes have Greek plants Prometheum tymphaeum and Sedum tristriatum?

Contandriopoulos, Quézel et Zaffran claimed that both 2n=16 whereas 't Hart claimed tymphaeum 2n=14 tristriatum 2n=22, 2n=44

29 May 2024 3,952 1 View

Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn?

Greetings! Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn? If did, which one and the details, please. Thank you very much!

29 May 2024 9,836 1 View

Hi everyone, please how do I map plasmids encoding ARGs on an assembled genome, and determine their transferability?

The result from PlasmidFinder shows that the assembled genome harbored many plasmids. Consequently, I want to investigate whether the plasmids are encoded for ARGs. And determine if they are on...

08 May 2024 7,467 0 View

What is the 'Effect' Column in GWAS Results?

Hello, I received some GWAS results. There is a column for SNP, position, REF/ALT allele, p value, SE, which is pretty straightforward. However, I am confused about the column titled 'Effect',...

03 May 2024 308 1 View

When using PGD the best perform it by FISH or by PCR ?

Which of this techniques can give us the best result in detection of chromosomal abnormalities

02 May 2024 1,205 1 View

Four SNPs on same chromosome, guidance about LD and haplotype analysis?

I am doing a research on pharmacogenetics of treatment of type2 diabetes. the SNPs i have selected are in close proximity on the same chromosome. I want to carry out linkage disequilibrium and...

28 April 2024 6,449 1 View