I would like to cultivate lactobacilli in an intestinal organ-on-chip model and stain it with a suitable dye either beforehand or, if necessary, after the end of the experiment with a suitable antibody for immunofluorescence microscopy.
Briefly, I would like to check the Lactobacillus attachment/localization to/in the intestinal tissue.
Is there anyone with experience in this area and could explain possible procedures?
Thank you very much in advance!