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Article Enzymatic hydrolysis during in vitro digestion of triacylgly...
Hi, I am trying to fit growth functions on several levels of a fish population (global, by habitat, by size at maturation) by non-linear mixed effects models (since the age/length data originates...
24 September 2019 7,380 2 View
is the bigger LRI (Linear retention index) or RI (retention index) means bigger concentration or peak area or percentage of peak area for GC MS measurements?
16 June 2017 3,071 5 View
I need urgent help in data collecting for my research in educational leadership to identify student leadership perception of teachers at schools. The measurement tool can be quantitive or...
12 May 2015 8,067 3 View
For bone marrow trephines we use a glutaraldehyd fixative, which for internal reasons is imperative. Decalcification in EDTA has been changes from overnight at 35°C to a stirred bath at 45°C, but...
08 February 2015 8,850 10 View
I was wondering if, due to high imbalance with dosages in long term illnesses of adrenal gland(like Primal Adrenal deficiency) and broad spectrum of side effects (especially hydrocortisone)...
26 November 2014 7,337 4 View
I need to do pairwise alignment of nucleotide sequences of length 15-200 nt. Preferably, it should be written in C and accept *char variables for the sequences.
26 June 2013 5,853 3 View
We work with lipids in milk and usually I prefer using SPE. Can you recommend other methods to isolate (preparative) monoglycreides from diglycerides and from triglycerides?
01 January 2013 5,765 5 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
En suivant toutes les étapes mentionnées sur (ajouter une recherche) il me signale erreur, ressayer, j'ai essayé plusieurs fois, sans succès, toujours il me signal erreur, ressayer
02 August 2024 9,719 1 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View