Ligninase and xylanase zymography's protocols

More Bita Zaferanloo's questions See All

Can anyone help me to find the concept of denovo sequencing and how we can do it?

Sequence protein

11 December 2012 574 2 View

Can anybody please suggest what is the reasons of having smear instead of separated bands in SDS-PAGE?

Smear in SDS-PAGE

08 September 2012 568 6 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

An optimal protocol of EVs isolation for proteomics?

Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...

17 December 2020 7,600 2 View

What can I use to dilute protein for gelatin zymography?

My protein sample is extracted with RIPA + PMSF ( Phenylmethylsulfonyl fluoride). After protein assay, the concentration is too high (>900ug/mL) for direct application for gelatin zymography...

13 December 2020 7,764 3 View

Why my samples stuck in the top of gelatin zymography?

I am trying to detect MMP2 activity through gelatin zymography. I do can see clear bands. But all my bands stuck in the top of gel. however the ladder passed through...also when i run the gel, the...

03 December 2020 310 2 View

Bita Zaferanloo

Hi Nando,

Thank you so much for your time.

I have done zymography for protease and amylase but still have a problem with running gel for xalanase and ligninase zymography.

I already read the second paper, for sure I will check all the papers and try again.

Cheers,

Bita

Veronica F.

Hello,

I can't understand in which way could I detect MUG fluorescence in the zymogram gels: Do I have to put gel on the UV device I usually use to detect agarose gels? or there is another way? HELP PLEASE :( thanks a lot

Vinod Kumar Nathan

you can view attached paper for xylanase and instead of xylan you must use ABTS for laccase.

Jessica Lenka

Have you seen any paper where a zymogram gel using ABTS for laccase was described?