Hellow everyone,
I work on optimization of lignin peroxidase enzyme production in MSM containing kraft lignin by bacterial isolates following this method:
• LiP enzyme activity was measured following veratryl (3,4-dimethoxybenzyl) alcohol oxidation to veratryl aldehyde. The enzyme reaction consisted of 500µL of 100mM sodium tartrate bufer (pH 3.8), 500 µL of 4mM veratryl alcohol, and 0.1mL of crude enzyme. To start the reaction, 0.1mL of H2O2 (2mM) was added to the mixture, and incubated at 30 °C for 5 min. A change in absorbance at 310 nm (ɛ = 9,300M/ cm) was observed.
The problem is that I always find the absorbance readings of control higher than that of the sample. which is opposite that found in literature that readings of sample must higher than control. did anyone do the same assay and find similar results? or what is the probable cause of those findings?
Thanks in advance
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