Ligation of SSR with competent cells with T4

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Mohammad Kabir Ahmed

How long the SSR is? Whatever it is. T4 ligase can be used at low temperature (4-10 degree celsius overnight.Try to use cohesive ends. Confirm the sequence.Multiply using DH10B competent cells. Electroporation is the best Culture in LB in presence of ampicillin, IPTG and X-gal. Pick white colonies and separately culture the independently in LB Liquid culture media. Confirm by sequencing.

Alshymaa Yusef

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