Ligation of spacer to Aptamer using DNA ligase?

More Ramya Chandrasekaran's questions See All

Can I use Doxorubicin treated cells for PI staining and FACS?

I want to do cell viability assay using FACS with Doxorubicin treated cells. Since Dox and PI absorbance wavelength is very close, I am wondering if there will be a cross talk between them?

08 September 2016 10,144 2 View

How do I confirm Silica etching?

I have a core shell nanoparticle containing gold core and polymer shell. There is an intermediate silica layer and I am trying to etch the silica layer using NaOH. My question is how can we...

09 October 2015 6,900 4 View

Can anyone recommend a CW laser system of 808 nm that I can use to treat rats injected with nanoparticles?

Can anyone recommend a CW laser system of 808 nm that I can use to treat rats injected with nanoparticles. I have a portable CW diode laser (Chanchung New Industries Opto electronics Tech.co. Ltd)...

02 March 2015 494 18 View

Is there a software for 3D drawing other than 3D max?

Can anyone suggest a software to draw 3D diagrams of cells, DNA etc? I have been trying to use 3D Max but I feel it is not user friendly (or maybe I am so ignorant with the software!)

10 November 2014 4,209 5 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View