I have an Aptamer to which I have to attach a polyT spacer that is 5' modified. Would T4 ligase work for this attachment? My concern is that T4 may not work for single stranded DNA?
It works... but with very low yield...
You gave very few details, but this may help you to have an idea.
http://www.ncbi.nlm.nih.gov/pubmed/16302964
Thanks Max and Andrei for the input..
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