Hello, I have a mix of 10 fragments generated with 2 endonucleases (1 produces blunt end and other , PSTI, produce overhang ends): each fragment has a blunt and and overhang end. I want ligate a 22mer oligo complementary to the PSTI end. Please how can I calculate the amount of each (oligo+restriction products)? Is it possible to ligate the oligo in single strand form or do I have to anneal it with the respective reverse complement ?
Thank you in advance