Dear all,
I've recently processed some samples for ATAC-seq. My corresponding ATAC-seq library looks different (see picture: Bio-Analyzer) than the expected profile. I was wondering if I can still sequence it or it will be too biased.
Thank you for your help
Best,
Karim
Sulagna Sanyal
Hi Karim! Did you get the answer? B'cause I got similar profile with my ATAC sample recently. It looked like the nucleosome peaks are not very significant and the only prominent peak was from excess amount of index primer. I was suggested not to proceed with this sample. It'd be really helpful if you can share your views on this. Thanks!
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