What are the chief physical/chemical factors regulating the handedness of cellulose nanocrystal cholesteric phases?
I am looking to predict some production for future onshore wind energy production in Portugal, is there any database with info of performances of the existing wind farms in the country? something...
28 July 2024 1,750 1 View
In most of the literature I am reading at the moment, the abbreviation μg m−2 h−1 is used to express mean annual fluxes of greenhouse gases. I am confused by the "h" and supposed it stands for...
27 June 2024 9,633 1 View
I have two antibodies that are staining well in IHC but not in IF for tissue sections, when using the same concentration and antigen retrieval method for both. I also know the secondary works well.
08 May 2024 3,890 4 View
Hello! I have tried to electroporate the Jurkat cell line with the Neon System using the parameters provided by the supplier, but almost all the cells die after the procedure. I have also tried...
10 April 2024 1,382 0 View
Con esta pregunta busco posibles mejoras en los programas de apoyo dirigidos a jóvenes en situaciones de riesgo. Para identificar cambios específicos que podrían aumentar la efectividad de dichos...
10 March 2024 9,580 0 View
Busco explorar formas de aumentar la accesibilidad a los servicios de salud mental para grupos marginados dentro de la población. Para así identificar posibles barreras que estos grupos enfrentan...
10 March 2024 7,192 8 View
Hi, I am currently working on my thesis and I am designing an online experiment with 2 factors, each of which has two levels. To briefly explain the experiment: participants will either see a...
08 March 2024 8,503 4 View
En la asignatura de psicología del desarrollo, del segundo año de la carrera de Trabajo Social hice junto a otras dos compañeras un trabajo en la que a partir de una película o serie debíamos de...
04 March 2024 9,929 1 View
Hice un trabajo en la asignatura de Trabajo Social Individual y Familiar, en el segundo curso de la carrera de Trabajo Social en el que hablábamos sobre la modificación de la conductas en...
04 March 2024 6,601 1 View
At the protocol I'm using now there is an added note indicating that it is possible to freeze the protein samples mixed with the laemmli to temporarily store them at -20ºC and denature them before...
20 February 2024 8,246 2 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View
I use X-ray diffraction (XRD) techniques to quantify the degree of crystallinity in my samples, referring to the following formula (CrI=Acrystal-Aamph/Atotal ). using deconvolution area methods (...
06 July 2024 6,234 9 View
Hello, I am looking for (estimated) degradation rates of cellulose in a classic anaerobic digestion setup (meso or thermophilic, WWTP sludge, 30+ days of operation) to have an idea of the % of...
01 July 2024 5,867 5 View
Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...
27 June 2024 9,652 2 View
To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?
20 June 2024 774 1 View
I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....
19 June 2024 6,404 4 View
I have to join tungsten wire to metallic plate with that adhesive and entire assembly has to be heated upto 600C in ultra high vacuum. I know about zirconia paste but it is not readily available...
18 June 2024 3,092 3 View
Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...
17 June 2024 8,552 2 View