How to find if Qualitative and Quantitative (discrete) variables have a relation?

I'm going to do a brief research about the dependance between the number of cases of Covid-19 and sex( male/female) . One of the requierements is to use inferential statistics to test the...

18 February 2021 7,092 11 View

Does anyone have a qPCR protocol for Lactuca Sativa?

I need to extract RNA, produce cDNA and do my measures via qPCR. How much material? Which stage of growth?

24 January 2021 559 2 View

Is PROCESS v3.5 by Andrew F. Hayes compatible with SPSS v 26 or 27 on Windows?

I installed the PROCESS Custom Dialog v3.5 by Andrew F. Hayes on two different versions of SPSS (26 and 27, both on Windows 10) running SPSS as an administrator and using the 'extension' menu....

30 November 2020 1,390 3 View

What are the digital competencies that university professors/trainers need to perform well & answer the demands of digital transformation businesses?

I would like to have access to academic research that compares the skills needed by university professors and corporate education instructors or facilitators. I would like to understand if the...

29 November 2020 442 3 View

Is there any research on in-vitro meat about sporadic cancerous cells and their possible impact?

The high rate of proliferation required of stem cells could produce genetic instability which may result in sporadic cancerous cells. Is there any research on this?

10 September 2020 3,666 1 View

Which is the best transfection method for cell lines that growth in suspension?

We are trying to transfect lymphoma cell lines, and we have tried several methods, the best is through the use of lentivirus of course, but in this pandemic days is very difficult to import this...

06 July 2020 8,043 12 View

Do you have in your country a regulation on sewage sludge in agriculture?

If in your country a regulation on sewage sludge for agriculture exists, please could me specify which microbiological parameters and limit values have been established? Thank you very much in advance

04 December 2019 8,690 3 View

Social media_systematic participation to giveaways?

I am analysing the outcomes of a pilot project involving people' reaction (reaches and engagements) to posts related to plastic pollution. To enhance engagement we proposed "challenges" i.e....

10 November 2019 571 0 View

Problems with RT-PCR of respiratory syncytial virus. Any advise?

Hello! I am having difficulty amplifying the coding sequence of RSV F protein. The protocol I have used is a sfollows: I infect HEp-2 cells with the virus, then when CPE is evident I collect the...

02 October 2019 3,061 3 View

Where does a single nucleotide ambiguity in sequencing of a digested plasmid, that was not in the original plasmid, come from?

We have been having issues with this certain plasmid for a while: we were trying to remove one gene (GFP) and replacing it with another. The digest of the original plasmid with BamHI and NotI...

11 August 2019 8,202 8 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How do I apply continuity boundary condition at the edge using form assembly in comsol?

The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...

05 February 2021 3,459 2 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Is carboxymethyl cellulose a better electrolyte host polymer than methyl cellulose and cellulose acetate?

I want to prepare a cellulose-based biopolymer solid electrolyte by solution casting technique. However, choosing the right cellulose derivative has been my major challenge. Among the many factors...

30 January 2021 5,355 3 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View