LDH isoenzymes.....

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Moaed E. Algazally Popular answer

Inhibition with urea method ....

Moaed E. Algazally

Heat stability method

Reaction with change substrate oxo-butyrate method

LDH-1 (myocardial LDH) is more heat-stable than that

of hepatic LDH (LDH-5).

Hepatic LDH (LDH-5) is inhibited by urea.

Cardiac LDH (LDH-1 and 2) utilizes oxo-butyrate as substrate preferentially to pyruvate; whereas liver LDH (LDH-5 and 4) has relatively low activity with oxobutyrate.

Maha F. Smaism

Thanks Dr. Moaed

Hadi Almosawi

By spectrophotometer absorption

and enzymetic method