Mention the chemical methods by which LDH isoenzymes can be estimated
Inhibition with urea method ....
Heat stability method
Reaction with change substrate oxo-butyrate method
LDH-1 (myocardial LDH) is more heat-stable than that
of hepatic LDH (LDH-5).
Hepatic LDH (LDH-5) is inhibited by urea.
Cardiac LDH (LDH-1 and 2) utilizes oxo-butyrate as substrate preferentially to pyruvate; whereas liver LDH (LDH-5 and 4) has relatively low activity with oxobutyrate.
Thanks Dr. Moaed
By spectrophotometer absorption
and enzymetic method
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