I use Shimadzu naxera LC-UVD. The mobile phase is (ACN:Water, 47:53) and wavelength is 220 nm. I try to run a expt with this parameters. But, unfortunately the baseline is not stabilized for long time. I try many wash with acetonitrile with a mobile phase (ACN:Water, 50:50), but still the baseline is not stable.

How can I fix this problem any troubleshooting? Please help me in this regard.

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