When a serum protein is used for development of a kit using semi-quantitative assay by lateral flow electochromatography, what other aspects need to be taken care of, for developing a kit successfully?
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I'm new to VASP and I'm trying to calculate the band structure of a bulk, nonmagnetic superconductor with a tetragonal crystal structure. I'm including spin-orbit coupling (SOC) in my...
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Gender, along with socio-economic and environmental factors, is a key determinant of vulnerability to climate change.
24 June 2024 8,437 2 View
am trying to make POSCAR file and xyz file using vasp for NbReSi compound using VESTA. However, I notice that the number of atoms for xyz file is given as 41 whereas for POSCAR is it 36. Can...
17 April 2024 698 2 View
Looking for papers on the same with detailed study, any help?
01 April 2024 6,592 0 View
I have seen nitrogen fixation in three heterotrophs, but cannot detect any nitrogenase in their genomes. Are there any alternative pathways that I can look into that may provide information about...
01 April 2024 5,641 0 View
In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...
31 March 2024 1,812 2 View
While Trying to manipulate end terminal carboxylic group protonation state in PET polymer chain, PSFgen package in VMD adds an extra -CH2 on the deprotonated oxygen. Also the chain is broken in...
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My study area is in eastern Himalayan foothill landscape which is a tropical to sub-tropical forest; elevation ranges from 150 to 1300 meter. The forest is some parts dominated by sal, tick and...
21 March 2024 8,297 1 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...
02 August 2024 4,751 3 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...
30 July 2024 2,950 0 View