How to interpret different leached phases of metapelites?

Extraction of three different phases of metapelites, mostly phyllites as the Carbonate phase, Fe-Mn Phase, and Silicate phase has been obtained. After doing ICPMS, different results have evolved....

11 February 2021 5,198 10 View

How to find out or construct(?) the right priors from data of previous experiment to use in a bayesian glm?

I have the following equation, Eating choice (Yes/No)~ Box type (A/B/C). So the question is whether a certain box type affects eating choice in groups of dogs. The eating choice of each member in...

10 February 2021 6,024 9 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

Why the bandgap of CH3NH3PbCl3 films are different for spin-coating method and electrospinning method?

The observed bandgaps are 3.8 eV and 3.4 eV for spin-coated and electrospun films, respectively. What are the possible reasons?

08 January 2021 5,646 6 View

Why monoethanol amine is used as flocculant?

I want to know the reaction mechanism of monoethanol amine used as flocculating agent for rerefining used lubricating oil. Here I have attached one research paper where MEA (monoethanolamine) is...

01 January 2021 5,681 2 View

Problem with GST-fusion protein elution- Can anyone help?

18 December 2020 4,559 6 View

How can I determine metal concentration of Used lubricating oil by AAS?

I am a junior research fellow in the department of Chemical Engineering, Nit Durgapur. I want to know that how can I determine various metal such as Fe, Cr, Mg, Mn, Pb concentration present in...

15 December 2020 9,887 3 View

Regarding the O 1s XPS pf phyllosilciates, the peak near 531 belongs to Oxygen vacancy sites or O bonded to the Si from the silicate tetrahedra?

In O 1s spectra, usually the peak near 531 eV belongs to oxygen vacancy sites in oxides, however, in silicates, these peaks are often reported to originate from O-Si. I am confused if the intense...

10 December 2020 9,496 2 View

What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

09 December 2020 1,863 1 View

How to PEGylate Fluorescntl Polystyrene Particle?

I have Fluorescent Polystyrene beads. I need to graft PEG on the surface of it. I am looking for a protocol to do that. Also need to know which experiment to perform to be sure about the grating.

01 December 2020 7,860 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View