I cant isolate lactobacillus from goat milk, i tried many times on MRS.
i don't think know
i'm asking about how to assembly a discret rigid part, please...instance cannot be created .
01 January 1970 7,833 4 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...
07 February 2021 7,520 6 View
Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...
01 February 2021 7,153 4 View
Does feeding affect heat in animal? if we were to add a specific feed (with data on dry matter content), how can we correlate feeding to estrus?
10 January 2021 9,719 13 View
I am trying to stain Marc145 cells infected with PRRSV. For this I am using an unlabelled primary antibody against N protein of PRRSV and using Alexa 546 as secondary antibody. After staining I...
15 November 2020 1,457 5 View
Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...
08 October 2020 4,647 6 View
I am doing immunofluorescence staining on retinal sections from RCS rats to assess GFAP. As shown in my attached image, the GFAP staining is not consistent with most literature I've seen with...
05 October 2020 8,105 3 View
Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...
19 September 2020 3,242 2 View
Any recommendations? I'm mostly (but not exlusively) interested in eastern Mediterranean and West Asia...
09 September 2020 7,344 3 View
