I am currently screening heat stress tolerant Lablab purpureus. One of the method is to quantitatively determine superoxide anion by using NBT staining method. I immersed the leaves in NBT dye for 8 hours, and was trying to remove chlorophyll by boiling the leaves in 80 celcius alcohol. It took me about half an hour to only remove the chlorophyll partially. Any suggestions how to remove it rapidly? Thank you.
Svetlana Boycheva Woltering
Hi,
I would try acetone but without boiling. It should only remove the unbound NBT so it shouldn't interfere with your staining. It will be more efficient than ethanol in removing chlorophyll though.
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