What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

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I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

What is the easiest and cheapest molecular magnet to synthesize at home and how can it be done?

im looking for a diy at home (home lab) molecular magnet in a chemical suspension that is stable at room temperatures and cheap

30 December 2020 626 1 View

What defines the time it takes to disconnect a molecular switch?

I have been thinking about a material that was made 6 months ago, which is a tetramethylsiloxane linked to a thiazolidinedione and finally to a naphthalene, but I can't find the original paper (if...

30 December 2020 5,347 1 View

Are there any molecular dynamics sofware easy to use for homo lumo interraction in molecular dynamics or some sofware for magnetic interraction spins?

I am working with molecular dynamics in a nanotube but I need easy to use software to be able to homo lumo, molecular dynamics and also charge densities in a chemical doping, I am also looking in...

30 December 2020 7,482 1 View

Vhb 4910 and carbon black as a electrode have some diference between carbon with pdms?

There is a dopant for the vhb 4910 actuator tapes that have acetylene black or carbon black which is carbon grease (carbon black + pdms) commercially, I am making a robotic actuator but I don't...

30 December 2020 3,144 1 View

Anybody with experience importing plasmids from USA to Saxony, Germany?

Dear All, I would like to import (through collaboration with an American institute) a plasmid. I work in an institute located in Leipzig, Germany and we would like to get a plasmid from an...

03 April 2018 7,636 1 View

Anybody with experience importing plasmids from USA to Saxony, Germany?

Dear All, I would like to import (through collaboration with an American institute) a plasmid. I work in an institute located in Leipzig, Germany and we would like to get a plasmid from an...

02 April 2018 8,359 1 View

What problems does SNAP tag for superresolution microscopy have?

I want to image cells without altering protein levels and going to do so by gene editing the proteins I am interested in. I was doubting between directly fusing the protein of interest with a...

31 August 2017 7,055 3 View

I run the copula between solar radiation data and sunshine hours for 2 stations. The result the i got was Frank copula and clayton copula.

May I know how to interpret these two copulas refering to my data? Hope someone can explain to me how to interpret my results? Thank you in advance.

20 June 2017 3,717 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View