Kits used in 16S rRNA Metagenomic sequencing?

More Alshimaa Moawad's questions See All

Any idea about how to apply ultrasound waves to a blood sample during its flow in test loop?

Hi every one I would be very grateful for your expert help Am planning for in vitro experiment in which an ultrasound waves ( frequency ranged between 1_50 MHz and pressure amplitude ranged...

06 February 2022 7,896 5 View

Useful mycology text book?

I recently started a project to investigate some murine mice models of C.albicans association and infection models. Reading original articles and reviews is quite helpful but I want to understand...

24 April 2021 4,825 11 View

RNA extraction from OG-500??

Did any body try to extract RNA from saliva samples collected with OG-500 for qPCR ?? unfortunately we are not able to reach the patients again , any ideas

18 April 2018 3,247 3 View

I need help with DNA extraction from saliva ?

saliva Samples were collected 13 months ago in OG-500 tubes (genotec) and preserved at room temperature and I started DNA extraction 3 months ago .the end point is to do sanger sequencing for...

10 January 2018 4,208 4 View

What tests can be done on a plant extract to detect yellow coloring matter?

I have a plant extract that has deep yellow color even when it is very diluted. What test can I do to prove that it may be used as a coloring matter?

02 March 2016 3,759 1 View

Make deaf in college more active

How can i encourage my students who are deaf more active,and also how i introduce subjects to them???

01 January 1970 4,788 1 View

How can I find Qiime2 bibliographic sources of updated commands?

I'm making my research on metagenomics and I want to find the source of the command 'vsearch merge-pairs' that used to be 'vsearch join-pairs'' How can I cite that?

28 June 2024 3,960 1 View

How to perform the anaysis of taxonomic on raw reads from the mixed sample?

Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...

09 June 2024 6,537 3 View

How to upload 16S and metagenomic data from fecal samples to NCBI SRA database?

Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...

03 June 2024 9,451 3 View

Good day all. Please who knows of anywhere l can send a liquid food sample for a shotgun metagenomic sequencing?

I am a Nigerian researcher working on microbial diversity of an African fermented food product. I will appreciate it if l can get a location and price for shotgun metagenomic sequencing.

20 April 2024 2,536 2 View

Need help with improving the 16S rRNA sequence quality with trimmomatic?

Hi all, I have just started to learn about bioinformatics and I need help with it. I have enriched some microbes from wastewater anaerobic sludge and sent them for 16S rRNA sequencing. Based...

29 March 2024 5,278 4 View

I got a low percentage of similarity (Below 80 %) for fungi by 18S rRNA mol. identification, Is there any need to repeat the process for crosscheck?

Do I need to repeat the process or can I go for morphological/ Biochemical tests for fungi identification?

21 March 2024 5,153 1 View

Sequencing depth and paired end reads length of 16s RNA metagenomics on water samples looking for low abundance organisms?

Does anyone have any advice regarding 16s metagenomics and an appropriate sequencing depth and paired end reads. Expecting around 10-15 organisms within the samples. Thank you :)

18 March 2024 636 2 View

How to download the protein sequences included in the CAZy databases?

In the latest CAZy sequence list (http://www.cazy.org/), over 3,600,000 sequecnes have NCBI accession and they can be easily accessed. However, the remaining 550,000 sequences only have a jgi...

13 March 2024 2,439 1 View

18S/28S band sizes in Tapestation are lower than expected?

I'm running RNA extracted from Saccharomyces cerevisiae in a Tapestation 4200. The RINe value is excellent, but the sizes of the 18S/28S bands are lower than expected: ~1000 and ~1800 instead of...

03 March 2024 7,681 1 View

Working on presentation about metagenomics and alternative approaches, what's approaches stands for here ?

full title: metagenomics and alternative approaches for the fundamental study and exploitation of telluric microflora "alternative approaches" here means metaproteomic and metatranscriptomics ?

03 March 2024 1,778 3 View

Abhijeet Singh

Check out the following link

https://emea.support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html

Secondly, 16S rRNA sequencing is not a metagenomics sequencing, it is an amplicon sequencing for metagenomics samples.

Samrendra Singh Thakur

I think you are asking about library preparation. You should use TruSeq DNA PCR-Free sample preparation kit (Illumina Inc., San Diego, USA) for library preparation.