Kits used in 16S rRNA Metagenomic sequencing?

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RNA extraction from OG-500??

Did any body try to extract RNA from saliva samples collected with OG-500 for qPCR ?? unfortunately we are not able to reach the patients again , any ideas

18 April 2018 3,246 3 View

I need help with DNA extraction from saliva ?

saliva Samples were collected 13 months ago in OG-500 tubes (genotec) and preserved at room temperature and I started DNA extraction 3 months ago .the end point is to do sanger sequencing for...

10 January 2018 4,202 4 View

What tests can be done on a plant extract to detect yellow coloring matter?

I have a plant extract that has deep yellow color even when it is very diluted. What test can I do to prove that it may be used as a coloring matter?

02 March 2016 3,698 1 View

Make deaf in college more active

How can i encourage my students who are deaf more active,and also how i introduce subjects to them???

01 January 1970 4,787 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Can someone help me identify the bands?

The samples are replicates (4) of total RNA from HEK-293 T cells. The ladder is a 1kBp ladder on a 1% native agarose gel buffered with 1x TAE. From my searches, there should be two sharp bands at...

15 January 2021 4,712 4 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Is it possible to transport rRNA native secondary structure through Nano channels?

if yes, does it consider microfluidic scales?

10 December 2020 5,286 2 View

RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites?

Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...

02 December 2020 1,310 2 View

Could anybody provide insights the best tools to create a custom database for taxonomy assignment of metagenomic data?

I am interested in the different tools that can be used to create custom databases for targeted sequencing and how to trim the databases based on the amplicon size? Also, should custom databases...

02 December 2020 3,105 3 View

Abhijeet Singh

Check out the following link

https://emea.support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html

Secondly, 16S rRNA sequencing is not a metagenomics sequencing, it is an amplicon sequencing for metagenomics samples.

Samrendra Singh Thakur

I think you are asking about library preparation. You should use TruSeq DNA PCR-Free sample preparation kit (Illumina Inc., San Diego, USA) for library preparation.