I need to provide me references about "Basin and Petroleum System Analysis" and "PetroMod Software"?

Dear Colleagues, I am glad to find you well and healthy. I hope to provide me any references, notes, lectures, or links that can help me making a complete and perfect course about the "Basin...

31 December 2020 6,531 3 View

How do I find the threshold of signal noise(really long narrow spikes) ?

Hi, I have an input EEG signal, I need to filter the data in Matlab, which means that I need to remove the noise that is the really long narrow spikes, the input data is attached in this...

16 August 2020 3,578 3 View

Does cardiac puncture damage the heart for protein analysis or histological staining?

Hello, we have been sacrificing mice and rats using cardiac puncture. I am curious if anyone knows of publications or has had personal experience relating to potentially altered protein...

31 July 2020 681 2 View

Can you suggest a lesson plan for teaching reading comprehension through gamification ?

Lesson plan for teaching reading comprehension using gamification based on Class Dojo website

09 July 2019 531 3 View

How I can get load-displacement curve that increase to the failure load and then decrease ( peak value )??

I made In Abaqus A model of Reinforced concrete Pile cap but when I try to plot the load - displacement curve the curve didn't decrease that I can determine the failure load exactly , so If any...

17 June 2019 8,248 3 View

Is sonicating cells for western blot necessary?

Once cells have been collected in RIPA, before centrifugation (16,000g for 20 min) should cells be sonicated? Does sonication improve protein yield?

14 May 2019 2,385 7 View

What is the purpose of heating RNA at 55-60 C when utilizing the trizol protocol during the last step when RNA is in water?

Does heating influence RNA quality? My understanding was the RNA should not be heated and kept on ice. Trizol protocol:...

13 January 2019 7,933 4 View

Does protein concentration change between freeze and rethaw for western blot??

Hello, My understanding is that protein from cell and tissue lysates degrade between each rethaw. Has anyone experienced differing protein concentrations, significant enough to affect loading...

09 December 2018 6,962 4 View

I Interested in count, how many objects on a Image, for example objects are fruits?

I have an image, with many fruits on RGB image. I don't have ground truth. my interest is, to count how many fruits on image using Deep Learning, which is the best way to count them. Is there any...

24 November 2018 6,203 3 View

Quadratic - multi variables regression ?

I have data with high co-linearity and I am trying to apply the multivariate regression using SPSS .I need to know if there is any method to perform multi linear regression for two variables which...

23 April 2018 7,658 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View