Does anybody here ahve any experience with immunostaining of KCNH2 (IKr) channel in hiPSC-CMs? Which primary antibody did you use?
I am working on Project To develop anhydrous crystalline polymorph of Pregabalin API. Standard theta value : Two –theta values are 9.55 ± 0.2 and 19.13 ± 0.2 need to be match and Melting Point...
03 May 2024 6,898 0 View
any one suggest updates for treatment of complex regional pain syndrome?
19 March 2024 2,107 5 View
I am working through the challenges of cell isolation from the rat heart. I am using VSMC media and endothelial cell media. In a homogeneous mixture of cells, would these media favor only the...
06 December 2023 5,007 1 View
Good Morning; Is it possible to identify specimens from this kind of images and are they acceptable for publications?
17 May 2023 8,708 1 View
How to effectively use and interpret the barium swallow: Current role in esophageal dysphagia NGM 2023 How to effectively use and interpret the barium swallow: Cur... I am senior author on this...
04 May 2023 2,329 0 View
I am using desmond in windows 11, How could I change from dummy GPU to local one?
29 April 2023 3,156 1 View
There are several health and physical problems, after recovering from the Corona virus, and I need research links on this topic, thank you very much for all
14 March 2023 3,672 5 View
Usually, mediators and moderators are tested in quantitative studies. However, can we test them in a qualitative study such as a case study?
14 July 2022 8,932 6 View
Answers from different disciplines are welcome.
11 July 2022 8,363 2 View
I am using ~ 400 nm polystyrene nanoparticles, and I would like to use very diluted particle solutions for my experiments (atto-Molar range and lower). After my experimental procedure, I would...
26 January 2022 794 8 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View