Karyotyping of fungal protoplasts by PFGE?

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Optimisation of multiplex PCR on cDNA template?

I want to detect gene expression by multiplex PCR. The multiplex PCR reaction works fine on genomic DNA, but on a cDNA template the same oligo mix seems not to work as nicely, although specific...

10 November 2016 9,821 6 View

Does someone know how to separate fungal protoplasts from intact cells by centrifugation?

Our fungus is yeast-like and doesn't form spores; filtration through meshes or filters doesn't work.

02 March 2013 2,240 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Leo J.L.D. Van Griensven

Sonnenberg, A.S.M., De Groot, P.W.J.,Schaap, P.J., Baars, J.J.P., Visser, J. & Van Griensven, L.J.L.D. (1996) The isolation of expressed sequence tags of Agaricus bisporus and their assignment to chromosomes. Appl. Environm. Microbiol. 62, 4542-4547.

Corrado Nai

Thanks, the use of EST might be a very good idea. I was wondering if there is the possibility to use differential voltages or running time, or restriction fragments?