Corrado Nai

3 Questions 8 Answers 0 Followers

Questions related from Corrado Nai

Optimisation of multiplex PCR on cDNA template?

I want to detect gene expression by multiplex PCR. The multiplex PCR reaction works fine on genomic DNA, but on a cDNA template the same oligo mix seems not to work as nicely, although specific...

11 November 2016 9,894 6 View

Does someone know how to separate fungal protoplasts from intact cells by centrifugation?

Our fungus is yeast-like and doesn't form spores; filtration through meshes or filters doesn't work.

03 March 2013 2,297 2 View

Karyotyping of fungal protoplasts by PFGE?

Does anyone have experience with pulse field gel electrophoresis (PFGE) protocols to determine chromosome number of filamentous fungi / yeast cell protoplasts? I found different protocols but I'm...

02 February 2013 8,607 2 View