I am looking for the activation of Jurkat T-cell and if you activate it then how can you measure their activation I could not find anything related to these following: CD69 IL2 The other question is how to inhibit activation (turning it on and turning it off?
Anything in relation to cytokines with Jurkat T cell.
If you could kindly direct me in any direction please, that would be amazing of you please.
Thank you very much.
Article Essential role of the adaptor protein Nck1 in Jurkat T cell ...
To measure the activity of T cells, you can :
1.measure CD 25 and CD 69 Vs. CD3.
2. Measure the level of IL2 secreted by the cells.
3. Measure the number of IF-gamma producing cells.
Hi Abdul,
I'm not sure what your context so hopefully this is not irrelevant.
In my experience, CD69 is a great way to monitor activation of Jurkat cells, and it's a just a quick and easy surface stain! I know people who looked at IL-2, which can be convenient when done by CBA - but in our hands not all Jurkat cells produce it. I should say that my experience is solely on Jurkat 76 cells. These cells are responsive to PMA+ionomycin activation, which quickly up-regulates CD69 (I looked as early as 2.5hrs). I'm not sure how you can. I'm not sure how you could block it though, potentially by washing it extenively or chelating Ca+? Good luck!!
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