Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice?
Greetings
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
Best method and number of embryos for extracting RNA from pig morulae?
21 July 2024 4,398 1 View
Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...
16 June 2024 2,715 4 View
Hello, I would like to inquire about how the expansion of the cumulus can be measured during or after IVM. Thank you.
16 May 2024 9,330 2 View
Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...
02 May 2024 2,548 0 View
We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...
25 April 2024 6,159 0 View
Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...
22 April 2024 8,178 6 View
I know it may be controversial, and I favour crediting the authors. However, knowledge should also be accessible to student researchers. My doctoral colleague and I would like to access the book,...
12 March 2024 1,333 4 View
I need to make my sample size calculation without a pilot study, my n=10, but how can I prove that I have this number?
02 January 2024 9,534 1 View
Dear Researchers, I am looking for ratios of sodium alginate (powder) and calcium chloride (flakes/ granulate) and processes to create calcium alginate in FabLab facilities. PS: I am doing my...
12 December 2023 6,435 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,156 4 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,027 0 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,933 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,032 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,706 1 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 725 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 761 1 View