Although we added proteinase k to one sample during RNA extraction after cell lysis, the ratio didn’t change . Now how can we know type of the pollution in order to pure the RNA?
The easiest way to do this is use one of the many kits for RNA purification on the market.
Dear Ahmad
Look at links below
http://tools.thermofisher.com/content/sfs/brochures/T123-NanoDrop-Lite-Interpretation-of-Nucleic-Acid-260-280-Ratios.pdf
https://www.researchgate.net/post/Why_are_the_260_280_ratios_higher_than_2_after_DNA_purification
https://biosci-batzerlab.biology.lsu.edu/Genomics/documentation/3130_NanoDrop_tips.pdf
I think your proteinase K did not work well.
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