Although we added proteinase k to one sample during RNA extraction after cell lysis, the ratio didn’t change . Now how can we know type of the pollution in order to pure the RNA?
Shen-An Hwang
The easiest way to do this is use one of the many kits for RNA purification on the market.
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Laurence Stuart Dawkins-Hall
Dear Ahmad
- google this question under my name and RG. I have written extensively on this subject
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Alireza Mordadi
Look at links below
http://tools.thermofisher.com/content/sfs/brochures/T123-NanoDrop-Lite-Interpretation-of-Nucleic-Acid-260-280-Ratios.pdf
https://www.researchgate.net/post/Why_are_the_260_280_ratios_higher_than_2_after_DNA_purification
https://biosci-batzerlab.biology.lsu.edu/Genomics/documentation/3130_NanoDrop_tips.pdf
I think your proteinase K did not work well.
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