Transformation of Bacillus subtilis?

Colonies appeared after transformation of pHT01 plasmid into Bacillus subtilis, and band was confirmed by performing colony PCR. However, if the colony is picked and grown (in LB+antibiotics) and...

27 October 2020 609 4 View

Is this correct molecular docking?

Hello, buds I need your help to interpret the attached data. R group in serine is CH2OH, but the data show the two O in the group with hydrogen bonds with a molecule. Is this correct?...

02 October 2020 7,787 6 View

Are there any corporations that have launched Enterprise MOOCs to the public?

I am studying Enterprise MOOCs and can't seem to locate very many empirical articles for this area. The model I am interested in is similar to openSAP (https://open.sap.com). Please share any...

28 September 2020 6,813 2 View

Dataset size and the need for cross-validation (medical domain)?

I am currently envisioning a deep-learning model that classifies pulse waveforms (normal vs. pathologic). At this time, the no. of pulse dataset is about 2,000,000, and the balance of normal and...

31 July 2020 6,713 3 View

[ELI5] FRET assay, Protease assay, Michelis Menten. How would you convert the fluorescence signal to the velocity (concentrion/time)?

Hello i am fairly new to the field of physical chemistry and only have a vague memory of it from undergrad classes. my FRET assay is set up like a regular FRET assay, so the enzymetic activity of...

18 June 2020 9,730 4 View

Do buffers need to match for DNA (aptamer) to protein binding in SPR on NTA chip?

Hello, As a newbie to SPR, I've been having problems with optimizing aptamer-protein binding on an NTA chip. My protein has a his-tag on it. Any help would be greatly appreciated. Question 1:...

14 June 2020 4,586 3 View

Stats test measurements ?

what stats to use for: 1. 3 metric dependent variables measured on one group of people in two conditions to see if the conditions effect the 3 metric variables (repeated-measures ANOVA?) 2. 1...

12 April 2020 4,221 3 View

How to prevent Au nanoparticle aggregation?

Hello all, I've recently been attempting to synthesize monodisperse diameter gold nanoparticles (approx 2.5nm) based on the work of Levi-Kalisman (2019). In short, particles are synthesized...

24 February 2020 9,127 2 View

How can I interpret this SEM image?

This SEM images are Li doped Cu2O thin films. Precursor of Li is LiCl(Lithium Chloride). I think there is no crystallinity. Is this correct?

31 July 2019 7,787 4 View

Is it possible to silver enhance a single antibody in a multpliex immunoassay?

Dear all, I have a slightly odd question regarding immunogold silver enhancement. I am aiming to look at 2 targets in the same tissue section, but I need a way to differentiate them using metal...

21 February 2019 7,817 2 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View