What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

How to phrase semi-structured interview questions using IPA without biasing superordinate themes?

Hello, I am preparing a semi-structured interview schedule for an interpretative phenomenological analysis study on marginalized individuals' career experiences as impacted by COVID-19. The aim...

19 February 2021 9,099 2 View

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19 January 2021 6,848 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

How much does radiative heat transfer effect thermal conductivity of materials and how to compare calculations to experirment?

I am calculating the phonon contribution to thermal conductivity for 4 perovskites (BaZrO3, BaSnO3, BaHfO3, and KTaO3) with relatively wide bandgap. When I compare my results for BaZrO3 to the...

27 December 2020 3,232 3 View

Proper method for assessing model invariance following Confirmatory Factor Analysis (CFA) and preceding 2x2 between-subject analysis?

I have produced a good-fitting, reliable baseline model according to CFA. The model contains several factors each loaded by several categorical (ordinal) items. I ultimately intend to analyze the...

15 December 2020 1,668 4 View

How to Reinstall Operating System for a TA Instruments 2910 DSC?

Hard drive crashed on an old windows 2000 that was used to control a TA Instruments 2910 DSC. Installed new hard drive and I am trying to figure out where I can find the software/operating system...

17 November 2020 2,467 3 View

Communities based websites do they serve a purpose?

I'm asking and posing this question as it forms a part of my final project year. I building an ASP.net community-based website coupled with an Oracle database and I'm looking for feedback,...

17 November 2020 7,852 1 View

How can I solve Error: "The coupling service failed to start" during simulation ANSYS 2Way FSI?

Hello, I am dealing with error during lounching 2 Way FSI in ANSYS Workbench. Setup of the Fluent is ok (stand alone I am receiving expected results) and FEM uses Transient Structural. Once I run...

11 November 2020 3,816 4 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View