We have studied the copper toxicity in Chaetoceros calcitrans in F/2 medium (without any modification) at salinity of 30ppt. But I have found in literature that modified aquil's medium is used for trace metal studies. In which they have used artificial sea water for the preparation of f/2 medium, to which chelator-metal solution is added. Further they they have reduced the trace metal concentration to f/10. Is this type of media modification is required when we are conducting the experiment in natural sea water?
Hi, I think you must use natural media b/c the behaviour of organisms different in natural than in lab, so you can use some natural resources such as sea water with some nutrient and yours copper as media with sterilisation and blank to compare with
Cheers
MoJo
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