Does anyone use ARPE-19s to assess fibrotic markers? If so, have they found the baseline levels high. Any advice on culture conditions and methods to induce EMT or alter their fibrotic protein profile are welcome.
Many thanks
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
25 July 2024 7,498 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.
18 July 2024 4,218 0 View
I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.
17 July 2024 5,633 4 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
14 July 2024 3,570 5 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hi all, I am trying to use mixed effect model to analyze my data, which including a baseline measurement for my exposure (A), and repeated measurements for the outcome (B). I do have some...
17 July 2024 8,682 3 View
I forgot to autozero during the run (Size exclusion chromatography.) and later i realised i forgot to do that and the baseline was not zero but below zero (and in some cases it above zero). I...
15 July 2024 5,551 6 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
I am trying to figure out how to demonstrate that I cannot induce expression of a specific gene in a cell line. However, the gene is not expressed at baseline, so baseline Cts are very high. This...
04 July 2024 5,232 4 View
I want to isolate and culture all immune cells from the adipose tissue to isolate EVs. What is the best approach to make sure all cells survive for at least 48h?
03 July 2024 6,501 1 View
I would like to know whether Education is still required in 2024 to earn money and survive? Many college dropouts are becoming millionaires in 2024. Does educational qualification even matter?
30 June 2024 5,145 4 View
I want to survive in this world without much use of my brain or thinking. How do I still survive in this world without using my brain?
27 June 2024 3,458 1 View
General understanding is that postbiotic particularly those that are heat-treated are stable to heat and their activity remains stable during gastric journey. Wondering if this is true for all...
25 June 2024 3,133 3 View
Most people take action and do some activity to survive in this world. How do I still survive in this world by doing literally nothing? How to become an activity minimalist?
19 June 2024 683 1 View