I would like to ask some questions about concentrations of Urea 3M and 8M in two experiments?

I would like to ask if anyone knows if concentrations of Urea 3M and 8M can interfere with protein purification at Ni-NTA and Glutathione Sepharose 43 resins. Another question, Urea, at 3M and 8M...

17 November 2020 5,014 3 View

I would like to ask if anyone among you knows what is the ideal concentration of TFA to use at a chemical synthesis of peptides?

During a chemical synthesis of peptides by the F-moc method, the following happened: all the initial steps of the synthesis occurred as planned, but in the cleavage, TFA is used. In the lab, there...

02 November 2020 2,997 8 View

Is there any software to simulate viral infection of cells?

Hello, I am looking for a computational simulator of in vitro infection of cells by virus, to be used for teaching purposes. Do you know any tools/resources ? I would like students to set...

13 April 2020 2,144 1 View

Does anyone has a good experience growing the TALL-104 cell line?

We bought this cell line 6 months ago and we are trying to make it grow, no success so far. We are using ATCC suggested media and replacing IL-2 every 3 days. Fully media changes (by centrifuging...

13 January 2020 7,719 1 View

EO771 mouse tumor transfection protocol?

Hello! I've been trying to transfect EO771 using lipofectamine 2000, but despite changing the transfection media after 6 hours of incubation I have a high rate of cell death. My untransfected...

13 November 2019 7,883 3 View

How can I calculate Ethanol's boiling point for a specific volume?

08 October 2019 7,724 3 View

How can avoid aggregation of cells in E. Coli staining with ThS and propidium iodide when trying to visualize them in an epifluorescence microscope?

I got competent cells of E. Coli induced with IPTG to express B-amyloid peptide and I stained them with ThS and propidium iodide after permeabilizated with Triton x 100; however, when I want to...

07 August 2019 3,158 1 View

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I have used FITC-dextran in my mice via gavage, and collected the blood samples to run together. Should I keep the samples at -20C or 4C? If I freeze my FITC can that affect the resulting...

31 July 2019 137 2 View

How quantify relic DNA in water samples?

I need to quantify the amount of relic/free DNA in water samples from a fish pond. I'm looking for a protocol with these method. Does anyone know of any accurate methodology for this...

21 May 2019 5,636 3 View

Which are the best resources to learn AnyLogic?

17 April 2019 7,086 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View