EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How do I reduce basal phosphorylation in PBMCs for phosphoFlow detection?

I'm measuring the phosphorylation status of different proteins (e.g. NFKB, ERK, AKT1, p38 etc) using phosphoflow cytometry. The protocol for surface and intracellular staining works well, all the...

22 July 2020 3,233 8 View

Is it allowed to apply Kubelka Munk function (1-R)^2/2R to Vis diffuse reflectance spectra on a glass powder?

Reflectance spectra are performed on the plane surface of a compact glass powder disk (5 mm thickness). The glass particles can range from 20 to 100 micrometers. The instrument is a portable...

21 July 2020 4,577 2 View

I need to do a top file or a prm file for Chlorogenic acid with an OPLSAA force field. Thus, how can I create these files?

I am trying to simulate the interaction between this acid and a protein, so I am following the steps of the protein-ligand Gromacs tutorial. However, I need a prm file to complete the complex...

27 November 2019 9,460 1 View

Transformation of an angular variable?

Which transformation should I apply to an angular variable (i.e. slope of a landslide) in order to normalize it (like arcsin to percentages)? Even though ANOVA are robust to not normal...

27 February 2017 8,585 2 View

Post hoc test for a contingency table NxN?

When I run a contingency table NxN and I get a significant result with the chi-square test, is there a post-hoc test in SPSS to address the comparisons? Or should I run logistic...

27 February 2017 2,858 1 View

Could you please provide references concerning the regulation of plant life cycle?

Wisconsin fast plants (Brassica rapa) are the product of a breeding program developed for decades. They grow to full size in about 28-30 days. What has changed in them respect to their original...

13 April 2016 3,638 18 View

Does proportion and percentage data, as CWM, need Hellinger transformation?

I have calculated one RDA analysis to taxa composition, and one to FFG composition using CWM method, vs environmental and spatial predictors. I understand that taxa abundances matrices need to...

15 January 2016 8,126 3 View

What prevents the matching of mRNA strands transcribed from dsDNA virus?

After the dsDNA nucleic acid enter the nucleus of the host cell it is transcribed from host DdRp II to form 2 strands of mRNA complementary to the 2 DNA strands of the virus nucleic acid. Does...

11 April 2015 367 2 View

Can any mathematician explain why this equation tends to 1/e, e=the Neperian base 2,71828319 ?

Working Pareto's distribution in Excel, I use the function P=F ^ [1/(1-F) ] to estimate the fraction P that receives more than one media. When F value approaches 1 from below, it results that P...

23 April 2014 7,316 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View