Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Langevin Dynamics in shear flow?

Hi, We are trying to simulate particles with Langevin dynamics in a shear flow by Lees-Edwards boundary conditions in MD simulations. It seems thermal forces and shear flow do not go hand in...

23 February 2021 1,761 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

How could I do a simulation for the energy consumption of a CNC machine?

I'm working on my dissertation and it is about how to save energy when a CNC machine goes to idle mode. (A concept model of the idle machine energy saving in industrial manufacturing...

26 January 2021 9,620 5 View

Is it possible basic matrix material having higher material removal it's corresponding composites materials In wire EDM process ?

Actually, I am working with Al-B4C- Al2O3 hybrid composites. I have performed the operation by utilizing the wire EDM process to my developed composites. I found alloy 6061 as a base matrix having...

21 January 2021 7,381 3 View

What are the reliable open source software for finite element analysis?

I am interested to work on the finite element analysis of materials behavior during machining operations.

27 December 2020 5,750 18 View

Advance Manufacturing Technologies?

I am looking some research papers about Advance Manufacturing Technologies.

07 December 2020 2,152 5 View

Why abnormal thrust force was generated in simulation of CFRP drilling?

I am doing some simulations of drilling carbon fiber reinforced plastics (CFRP) with Abaqus/Explicit. 3D Hashin's damage model with damage evolution has been defined for the CFRP composite. The...

20 November 2020 6,715 1 View

Why thrust force, temperature and surface roughness increase with feed rate in Machining?

Reasons why thrust force, temperature and surface roughness increase with feed rate in Machining?

17 November 2020 873 4 View

What are good research topics in educational data mining?

I'm in the process of starting my masters thesis, and I would like to research on Educational Data Mining (EDM) area. I need good topics for research EDM. Can you suggest some topics? I'm working...

07 September 2020 7,535 4 View

For steel, are the qualitative measures of machinability and toughness, directly opposed to one another?

When I think of a machinable steel, I think of a steel that lends itself to small chips (easy chip breaking) during a machining process such as lathe turning. On the other hand, 304 stainless...

15 August 2020 3,959 10 View

Which gas-liquid mixture can be considered as a feasible medium for near-dry electrical discharge machining?

11 August 2020 6,657 2 View

What is the best Free/Open Source software for doing Taguchi and other design of experiments and associated meaningful analysis like ANOVA?

Also what are the different efficient DOE and Analysis methods w.r.to machining operation with a specially sintered tool?

03 August 2020 4,553 3 View