Enzyme purification The first step after dialysis is to purify the enzyme by ion exchange technique, where it appeared for me three large peaks representing three isoenzymes and some small peaks. Samples representing the isoenzymes of the enzyme that were purified by gel chromatography showed three distinct peaks representing the isoenzymes with the disappearance of the peaks The other small ... Question: - Is this result considered reasonable and can it show three peaks for isoenzymes in the gel chromatography technique?
Hi there,
Generally isoenzymes are very close in size and differ in their primary sequence. Therefore IEC is the most appropriate approach to solve an isoenzyme mixture as the overall charge of a protein depends on its sequence.
My question: - Is it possible for more than one peak to appear in gel chromatography in proportion to the number of peaks that appeared in ion exchange chromatography for the same enzyme?
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