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Enzyme purification The first step after dialysis is to purify the enzyme by ion exchange technique, where it appeared for me three large peaks representing three isoenzymes and some small peaks....
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Purification was carried out for a specific enzyme. I used the ion exchange technique first. It showed three peaks that represent three symmetries. Then these symmetries were taken. The three...
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My question: - Is it possible for more than one peak to appear in gel chromatography in proportion to the number of peaks that appeared in ion exchange chromatography for the same enzyme?
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Is the appearance of a single peak in gel filtration chromatography when purifying an enzyme with several isoenzymes necessary, or is it possible that more than one peak appears?
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