How it is possible that DNA primers previously working suddenly stop to worked?

I have used the same exact sequences in primers while ordering the new primers. The old primers are that I used previously which was worked successfully. Similarly, the sequences in tubes are also...

20 August 2020 3,132 12 View

How to perform Reciprocal Space Mapping (Symmetric and Asymmetric) XRD for oriented thin films?

Let's suppose I have an STO substrate and I have deposited a thin film of another material and it is highly oriented and STO as well as my material both form are cubic. To better understand the...

14 May 2020 6,359 3 View

What is the range of maximum DC link voltage on the grid connected solar system at present? Why we need to go for higher DC voltage?

What is the range of maximum DC link voltage on the grid connected solar system at present? Why we need to go for higher DC voltage?

19 April 2017 7,550 3 View

Info on fluorescence excitation and emission?

Hi, i am working on fluorescence microscopy..i am trying to build a small microscope to study fluorescence (eGFP) ..I would like to know if there is any limitation on the excitation time and...

14 April 2016 1,210 9 View

How do I generate optical delay?

I would like to generate optical delay of around 50 ns. But I dont want to increase the path length a lot, nor can I induce multiple reflections, since that would reduce the intensity of outcoming...

11 September 2015 4,196 18 View

Why is proton stripping reaction more favorable as compare to neutron stripping?

Please explain or suggest where I will get this answer?

20 January 2015 3,739 7 View

Does any one know updated ribosomal databases?

We have used rdp data analysis for ribosomal RNA gene (rDNA) amplicon reads from high-throughput sequencing (sanger sequencing). Almost half of my data was categorized as unclassified. I thought...

11 November 2014 6,977 2 View

How can I construct an extra column in SAP2000 with gridlines?

I am currently modelling a monument. In it I have to construct a extra column and beam, but after providing frame structure the gridline option goes off. So how to construct column after that?

11 June 2014 8,814 3 View

What decides the preference of any basis set used in a method?

Is it true that choosing a basis set is not a science but an art?

20 April 2014 5,385 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View