Is this mycoplasma contamination?

More Pratik Ramjivan Shriwas's questions See All

How the electron and hole pocket near the fermi level in the band structure affect thermoelectric properties of Topological half-Heusler compound?

How the electron and hole pockets near the fermi level in the band structure affect the thermoelectric properties of the topological half-Heusler compound?

26 June 2024 2,933 0 View

Is there a good way to find ZZ symmetric basis for 1D Quantum Lattice?

Hello, I am working with a Hamiltonian with ZZ conservation symmetry. From what I know, if a symmetry is known for a given Hamiltonian and we can find a basis for that symmetry, then we can,...

28 March 2024 3,570 0 View

I am interested to learn Transfer matrix method for perovskite solar cell. Any suggestions how to get a knowledge of this?

Theoretical method for perovskite solar cell

12 March 2024 9,329 0 View

Is MOE a reliable software for molecular docking?

Schrödinger is one of the most prominent software for molecular docking. Is MOE also reliable for ligand docking. regards, Pratik

06 September 2023 871 3 View

How to do the gromacs clustering of NAMD based MD simulation output please suggest ?

please provide codes

25 June 2023 809 2 View

How to make hydrophobic silica aerogel without aggregation of silica particles?

i am trying to to reduce the particle size of silica aerogel but while reducing the particle size the particles of the silica aerogel aggregate..please give me somew suggestion

19 April 2023 9,444 2 View

How to check Cytokine level in Rabbit after wound creation?

I want to check Cytokine (IL6 ,TNF-alpha ) levels in the Rabbit after wound creation with and without treatment . I am planning to do ELISA quantification. As per my research Blood plasma would be...

12 April 2023 1,053 1 View

Hi Can anyone send any tutorial how we can plot electron density map of different Miller planes of a particular crystal structure by G Fourier ?

Is it possible to plot electron density map of different Miller planes of a crystal structure

12 December 2022 3,408 1 View

Anyone knows Microbiology test specification for Cosmetics?

Anyone knows Microbiology test specification for Cosmetics? need for TAMC, TYMC, Pathogens specifications

10 November 2022 9,532 3 View

Carbon input by cotton and greengram ?

When calculating C input, how much rhizodeposition by cotton and green gram root residue should be taken into account?

31 July 2022 3,212 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Vilma Petrikaite

It seems that your cells might be contaminated. I would suggest you to use PCR reaction to detect mycoplasma in order to be sure.

Badreddine Mechri

Hi,

I'm totally agree with Dr. Vilma, it's clear that your cells are contaminated. I recommend this article, I hope that will be useful. Good Luck.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584481/

Cecilia Bucci

Unfortunately your cells look heavily contaminated. To investigate whether this is a mycoplasma contamination there are commercial mycoplasma detection kits based on PCR or antibodies.

Abdelkader Mohamed Elsayed

Nice Contribution Vilma Petrikaite