Any ideas to improve (text) Search engine based on User feedback on search results?

Hi Everyone, I have a documents search engine, and the users have ability to Rate the search result for any query they make. For the first versions of Search engine, I am using Universal...

26 October 2020 7,557 10 View

In IR systems, what is the preferred measure if we have incomplete relevance data? MAP, nDCG and bpref_R or any others?

I want to setup a search engine performance system to automatic estimation. Say we have a dataset with more than 30,000 documents, its hard to find relevance data for some queries. which measure...

27 August 2020 3,152 6 View

How to calculate Qmax value from Langmuir Equation?

I am doing dye removal experiment and need to calculate Qmax value from Langmuir Equation, Kindly help me with example .

08 June 2020 8,909 1 View

How can I specify the viscosity of the input flow in simulation on Non-Newtonian fluid in ANSYS fluent ?

I am new to ANSYS fluent. I am trying to perform simulation of Non-Newtonian fluid in ANSYS fluent. ( Shear rate dependent) I was able to tune the consistency index( k), power-law index ( n), and...

04 May 2020 3,484 2 View

Does type-II junction possible for Ohmic metal- semiconductor junction?

My device shows linear current-voltage characteristics. It also shows highly stable photovoltaic behaviour. Please give me appropriate reason and reference (if possible).

22 March 2020 6,226 5 View

Metabolite set enrichment overview?

Hi, I have input data in Metaboanalyst in MS peaks to pathway module. I was able to find the graph as attached (input figure 1). I also generated the report. But I am not sure how would I get a...

14 January 2020 2,631 2 View

How to do endosymbiont analysis in insects?

I have sequences of 16s bacterial specific polymerase chain reaction from bollworm so how to do analysis what type of study can be performed please explain

19 November 2019 8,823 4 View

What should be the dispersion liquid for determining the zeta potential of graphite oxide and graphene oxide ?

I have used refractive index of 1.96. And distilled water as the dispersion liquid. And a zeta dip cell is used. Each time the value of zeta potential is showing zero irrespective of pH. Also,...

13 March 2019 6,178 5 View

Crispr ko cell line?

Hi, I generated a crispr ko cell line. The protein was more than 90% knocked out. But, now after several months od thawing and repassaging,the protein seems to comeback. I plan to do a grloth...

11 January 2019 3,904 9 View

What is the best way to detect and eliminate mycoplasma contamination in cell culture ?

I am doing some experiments on virus infectivity. My positive control cells did not support strong virus replication. The cells in culture look very unhealthy after 2 days and I always make sure...

10 January 2019 3,175 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View