Hi, guys, I am a postgraduate with lots of experience in culture contamination. However, when I was about to be sure I will never be bothered by contamination, this (as is shown in the pic) occurs！ and spoil my almost one-month effort........I dont know what this is and where they may come from, can anyone give me some help？Please！
Thank you guys for your answers! After several days of observation, yellow objects grew more in number, and there are more and more dead cells in the culture. Some objects are floating while others in inserted between cells. From my point of view, they do not seem like cell debris or any other "healthy" things..........
Hi Yuan,
What kind of cells are and what culture medium are you using? In the image seem crystals, perhaps salt deposits that could proceed from precipitation of components of the culture medium? It has been used many times this bottle of culture medium?
Here is another possibility. It might not be contamination with an organism or virus. When you disperse your cells with trypsin to subculture there may be some small clumps of cells passed to the new flask. Incomplete dispersal. The cells in the clumps die but do not affect the growth of the other cells. I would have to see the actual cultures to be sure, but make sure that your trypsin/EDTA (or whatever you use) is new.
A follow-on possibility related to Peter M Bradley’s information is the possibility that foci (aggregations) of cells of cells have accumulated and may either grow on or detach from your culture substrate.