When lactobacillus is inoculated in MRS medium, it appears as lysed cells. The aspect of colonies on MRS agar plates is normal. Is it a sign of contamination?
Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...
07 February 2021 9,791 2 View
Hi, I am working on 48V Lithium-Ion battery pack. I am wondering if there is fast MOSFET (or any other technology), to control the current of charging and discharging the battery with high...
20 January 2021 1,717 4 View
Hi! i am running a simulation using a cluster and I have encounter an error during my nvt equilibration like this: Cannot rename checkpoint file; maybe you are out of disk space? I deleted some...
03 January 2021 3,544 2 View
I have coated the Silicon wafer with photoresist (AZ1518) but until the exposure (Laser Writer) I had to wait for more than a week (Laser Writer was not operative) - would the photoresist still be...
03 January 2021 9,640 4 View
The DNA molecule is in the form of a double helix, The atoms which compose it are carbon, nitrogen, hydrogen, and phosphorus
02 January 2021 4,402 3 View
Modification of the rheological properties of asphalt
25 December 2020 7,390 2 View
I am trying to work on reducing the PAPR of the FBMC/OQAM with the TR and PTS methods. for that if someone can share with me one of these matlab codes. my e-mail: [email protected]
19 December 2020 7,764 1 View
How to estimate the acidity of a cheese?
13 December 2020 4,890 4 View
I am going to clone gDNA fragment > 10 kb into a pCR TOPO vector. I performed the PCR using KOD PLUS Neo polymerase for 3-steps PCR reaction, I am not able to find the specific size. Several an...
03 December 2020 1,939 5 View
I always found confusing both concepts! Which one do you think is more important? I give an example: The journal of "Theoretical and Applied Climatology" has an impact factor of 2.88, but it's a...
01 December 2020 8,608 19 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...
23 February 2021 7,868 2 View
Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...
22 February 2021 6,126 2 View
Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...
13 February 2021 4,040 3 View
I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...
12 February 2021 3,926 3 View
Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...
07 February 2021 8,258 3 View
Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...
02 February 2021 7,892 4 View
Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...
01 February 2021 7,153 4 View
Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate
01 February 2021 9,509 1 View
I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...
18 January 2021 5,151 1 View