Is this culture of lactobacillus is contaminated ?

31 May 2022 0 4K Report

When lactobacillus is inoculated in MRS medium, it appears as lysed cells. The aspect of colonies on MRS agar plates is normal. Is it a sign of contamination?

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Ameni Ben Ammar's questions See All

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

Thermophysical properties of MWCNTs nanoparticles?

Is there any article can I find all thermophysical properties of MWCNTs nanoparticles especially, thermal expansion coefficient and electrical conductivity?

30 June 2024 4,075 2 View

Exergetic effeciency analysis?

How to calculate the the exergetic effeciency of heated pipe with a constant heat flux?

30 June 2024 1,039 1 View

Please can you help me identify this specie of Euphorbiaceas family?

I found this ornamental plant in the garden please help me identify this specie.

30 June 2024 4,499 8 View

Are there recent publications on the economic comparison of Solar PV Curtailment vs. BESS Installation?

I'm currently exploring the economic implications of solar PV curtailment versus the installation of battery energy storage systems (BESS). Specifically, I'm interested in recent publications or...

27 June 2024 6,423 2 View

I need content writers, poets, researchers to my new online magazine, would you be interested?

Good day! I am the CEO of an online magazine called Order Of The Pen . We specialize in promoting new writers via our platform on Wix and Partnership with Mailchimp for email marketing,...

18 June 2024 4,117 0 View

3T3-F442A cells not adhering to coverslip?

Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...

16 June 2024 2,754 0 View

Fluid flow through copper tube?

What does it mean meshed wall and shell conduction with or without run-out method use to meshing copper tube in Ansys fluent?

07 June 2024 9,030 3 View

Can I find a fast journal (indexed in scopus and without fees)?

Good greeting. I want to publish a paper review in a journal specialized in food, health, toxicology, or even environmental sciences, provided that it is indexed in Scopus. I hope you can help...

19 May 2024 4,426 4 View

How to plot in Simu5G on Omnet++?

Dear network I need to plot instantaneous and mean End-to-end delay in D2D and eNode-based topologies? How to generate/visualise the accurate vec file data? I appreciate your help

14 May 2024 2,897 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Can we add calcium carbonate to any media as an indicator for lactic acid producing bacteria?

As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...

20 July 2024 7,504 0 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Black dots like contamination in my cell cultures. What are they?

There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...

11 July 2024 4,829 0 View

Does the soil contamination correction factor for decomposing leaf biomass vary according to space and season?

The question refer to leafy biomass decomposition using litterbag techniques.

11 July 2024 2,078 0 View

When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?

07 July 2024 5,480 2 View

What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?

02 July 2024 9,204 0 View