Distinguishing between human activated and exhausted lymphocytes T?

Any suggestions on how to distinguish between activated and exhausted human lymphocytes T CD8 with flow cytometry? It is known that exhausted lymphocytes T have higher expression of inhibitory...

28 December 2020 255 3 View

Help with lentiviral vector?

Hi everyone, I am trying to produce eGFP lentiviral vector by transducting HEK 293T with calcium phosphate method. I transduce cells when they are at 70 % confluent (following this method...

14 October 2018 2,351 4 View

Cross-sex genetic correlation

Hi! We are trying to estimate body mass (W) heritability and cross-sex genetic correlation using MCMCglmm. Our data matrix consists of three columns: ID, sex, and W. Body mass data is NOT...

25 July 2017 6,702 1 View

Which are the best melanoma cell lines?

Hello, I'm working on melanoma and I'm searching the best melanoma cell lines (easy to culture) recapitulating all the steps of melanoma growth: Radial growth-phase cell line, vertical...

05 June 2017 8,061 2 View

I would like to extracte cell membrane from frozen buffy coat. Can anyone suggest a reliable protocol?

Hello everyone, I need to isolate cell membrane of leukocytes and platelet, from frozen buffy coat. Any suggestion?

20 September 2016 5,749 1 View

Fibronectin antibody clone FBN11?

I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...

30 June 2016 1,973 2 View

Does the rule of choosing a component if the model performance increases by >5% measured by RMSEP (in cross-validation) apply also to PLS technique?

When choosing a WAPLS model, apart from relying on performance (e.g. r2, r, max bias, RMSEP, scatter-plot etc..), the recommendation is to select the successive component if the model performance...

15 May 2016 1,200 2 View

How can I concentrate down some digested liquid coming from in vitro digestion without disrupting its micelles?

20 April 2016 3,381 3 View

How can I effectively debenzylate a tertiary amine?

31 March 2016 9,050 8 View

Multiple choice feeding experiments - Can anyone help me decide on my statistical approach?

I performed a multiple choice experiment to assess the food preference of the sea urchin Paracentrotus lividus in relation to 5 different food items. I have 15 replicates, each represented by a...

03 February 2016 8,060 4 View

No title

02 March 2021 324 2 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How to select from various invitations from Scientific Boards in conferences and proceedings?

How to select from various invitations from Scientific Boards in conferences and proceedings? How to spot the ones with high impact and genuine? Can we spot valuable events?

02 February 2021 8,187 1 View

What is the most appropriate design (statistic test) to assess the toxicity of 2 different compounds at 6 different concentrations and 2 conditions?

10 January 2021 8,077 3 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Is it easy to collect a conference certificate?

share your experience please.

01 January 2021 5,084 4 View

Is training really prepare you for a conference?

Does training really prepare you for a conference?

01 January 2021 1,601 2 View

What is the procedure of attending international conference and seminar?

What is the procedure of attending international conference and seminar?

01 January 2021 7,733 1 View