Hi,
My HCC1937, MDA-MB-231, and BT-549 cell lines have all shown signs of infection 24hrs after waking up from LN.
The images attached are from the same flask of HCC1937s.
Would anyone have suggestions as to what could be done to prevent this? I had used the same TC hood as a colleague straight after her, and my cells showed infection while hers did not. I therefore, don't think it is related to the TC room, nor the TC hood.
Any suggestions?
It seems like that
use filter tips and filtered stripettes
package primary cell cultures with plastic wrap, it allows free exchange of CO2, but blocks any contamination, Avoid using the waterbath., sterifilter everything into autoclaved bottles.
The images were not clear, many methods may be used, depended on the isolate type.
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