I was thinking of a way to measure enzyme levels in serum, or other fluids for diagnostic purposes, especially in third world countries. Enzyme activities like lipases, liver enzymes, and other enzyme activities could be measured, possibly with a hand held device and would be cheap and easy enough to sell over the counter.
why don't you try to correlate enzyme levels with protein content? Bradford or Lowry methods are quite cheap & convenient.
I was thinking along the lines of quantifying enzyme activities like lipase, liver enzymes, and cardiac enzymes for diagnostic purposes. The test would be so easy, that it could even be sold over the counter.
From my personal experience with lipases, I 've found that an easy technique is to quantify activity using p-nitrophenyl palmitate as substtrate, measuring the paranitrophenol that is being released using a spectrophotmeter.
In general you would need the substrate, which you can easily find in the 'usual' industries, paranitropenol for standard curves to correlate the performance with something, a protocol to extract the lipases (usually detergents and sonication), and a spectrophotometer.
I don't know if it sounds too complicated, however it takes about 5-6 hours for the whole procedure, and the results are convenient.
I hope this helps.
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