Is there specific in-vitro proliferation markers for retinoblatoma and Uveal melanoma (monitoring growth)? What are they?

More Ahmad Samir Alfaar's questions See All

Using OBD technique i am trying to measure laser induced shockwaves velocity i found that at start velocity increases and then decay?

i am unable to interpret why its increases in start as shown in figure

11 August 2024 2,179 1 View

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

To what degree does the immunohistochemical (IHC) evaluation of Ki-67 hold diagnostic value beyond neoplastic pathologies?

In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...

25 June 2024 7,825 2 View

Problem with nucleofection RNP delivery to murine primary T cells?

I have been using the Lonza nucleofector for RNP delivery to OT-I T cells without any issues for the past six months. However, recently, my nucleofected OT-I T cells have not been proliferating in...

13 June 2024 2,279 0 View

Primary salivar tumors in bone?

Although rare, some salivary tumours can grow primarily in nearby bones instead of salivary glands. The diagnosis of said tumours is defined by Batsakis criteria (1979). I would like to know if...

10 June 2024 8,967 2 View

Hi all, could anyone please suggest me how to do Data Analysis of Cell Proliferation Assay using Flow Jo Software in Flow Cytometry ?

Its an In Vitro Cell Proliferation using Leukemia patient cells lines. Cell Proliferation Dye Used : Tag-It Violet Cell Proliferation Dye. Thank you a lot.

09 June 2024 1,194 1 View

What is the difference between self-renewal and proliferation?

A stem cell can divide to produce 2 identical copies of itself, which is called self-renewal. Then what is proliferation in terms of stem cells. Or is this term 'proliferation' applicable to stem...

04 June 2024 6,245 4 View

What is the cause of HL-60 cells froming aggregation?

HL-60 cells are cultured and expanded in IMDM medium containing 20% FBS. However after thawing I observed that these cells made disordered aggregates although they are still being proliferated and...

30 May 2024 2,722 0 View

Is CFSE retained in cell nuclei?

Does anybody know whether CFSE is retained in cell nuclei after treatment of cells with pepsin solution in HCl?

29 May 2024 1,357 0 View

Why CD45+ cells die during the course of expansion?

Hi all, I am working on a project which needs expansion of CD45+ selected cells from tumor and lymph nodes. My Tumor samples expand good but my samples from the lymph nodes are less and less...

23 May 2024 2,839 0 View

Need assistance in optimizing my protocol. Anyone can assist me about inducing hPSC or iPSC to MSC?

Dear Colleagues, I am currently working on a project that involves differentiating human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs)....

19 May 2024 3,164 2 View

Filippo Mancuso

Hi Ahmad, do you have melanoma cells culture? Or a 3D culture with melanoma cells ? In the first case you can just use a XTT proliferation assay; In the second case, you should firstly mark melanoma cells for a specific melanoma marker such as HMB45 or Melan-A.

Kind regards,

Ahmad Samir Alfaar

I have just found the following papers that compares the markers in each cell line: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3288394/

But what is the case for Retinoblastoma?