What is a good marker for imaging mouse macrophages by immunofluorescence in FFPE lymph nodes?

I am new to using mice, and I would like recommendations for specifically staining most, if not all macrophage subsets in murine tissues, particularly in lymph nodes and spleen. My background is...

16 November 2020 3,412 3 View

What is the current status of EOS modelling in unconventional reservoirs, esp. those with uplift and secondary migration of high-maturity gas?

Equation of state (EOS) modelling in CONVENTIONAL reservoirs is well established and has been successfully employed in PVT studies of basins globally for many years. However, what is the current...

12 November 2020 5,153 1 View

Can I image AlexaFluor488-labeled proteins in fixed tissue?

I'm planning to administer an immunogen labeled with AlexaFluor-488 into macaques with the goal to image the immunogen in the draining lymph node after it is biopsied. I'm having trouble finding...

21 September 2020 7,156 3 View

How to calculate sample sizes for multiple logistic regression?

Dear friends, I am looking for a procedure to calculate sample sizes/evaluate the power in logistic regression models with 3+ predictors which are partly binary partly continuous variables. Can...

03 October 2019 5,336 5 View

How do I analyze Likert scale questions in my dataset?

Hi, I have a data set in SPSS with several Likert scale questions. I wanted to see if there are any differences with how males and females answer several satisfaction questions (e.g., satisfied...

19 August 2018 1,554 4 View

Does resistance have an upper cross sectional limit where the proportional definition of resistance and resistivity breaks down?

I have seen evidence of a lower limit of this equation, but want to determine the resistivity of a composite semiconductor that has a cross section on the order of 100's mm^2 and about 100 mm in...

28 November 2017 8,453 2 View

Is there an open data collection of news headlines?

Some of my colleagues are planning to analyse news headlines related to climate change. Does anyone know of a good source for historical news headlines as open data?

08 September 2017 8,320 4 View

In alkaline comet assay is it possible to use normal melting agarose diluted with milli-Q water, or you have to dilute it with TBE?

Our slides coated with NMA with TBE always get cloudy after drying. We are using alkaline comet assay to evaluate sperm DNA fragmentation.

11 December 2016 2,823 5 View

Whatever happened to psychosemiotics?

I have only recently stumbled over psychosemiotics as a specific field of research and was struck by how much its concerns match my own interest in applying evolutionary psychology and certain...

02 March 2016 7,453 0 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

How to address mutual causality using Propensity Match Scoring using a panel dataset?

Hello dear researchers. I have a panel data set where I regressed innovation on director tenure. Though, I employed GMM and lag of the director tenure to address the endogeneity and reverse...

08 February 2021 5,809 1 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Hello, can you recommend a method to remove serial correlation from path coefficients (standardized regression coefficients)?

I would like to measure the causal effects among variables in a path model, but the variables (time series) may be serially correlated. I'm looking for a method to remove or otherwise manage the...

02 February 2021 210 3 View

Cross-lagged panel analysis and sample size?

I want to estimate the causal relationship between two latent variables over time using structural equation modelling. both latent variables are measured according to different indicators...

31 January 2021 3,777 5 View

Without using a course aggregate why the topic is named as Textile Reinforced Concrete? & why not it is named as Textile Reinforced Mortar Cement?

Dear Researchers, In Textile Reinforced Concrete is a novel construction material consisting of a cementitious binder and a textile reinforcement. Without using a course aggregate why the topic...

24 January 2021 2,654 9 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View