No title

Hi there! I'm very new to r and trying to run a multi-level meta analysis using pre-calculated effect sizes. I've attached the datafile below. The script I used as a first step to fit the model...

12 January 2021 8,214 2 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

Can hypoxic air (1% O2, 5% CO2, 94% N2) buyed from the market?

I need hypoxic air (1% O2, 5% CO2, 94% N2) for hypoxia experiments, can I get/buy some hypoxic air from the market?

14 July 2020 5,471 2 View

Need help- how to use primary and secondary data in same paper in regression?

Actually am trying to use regression for my paper where am drawing primary data questionnaire(independant)from few universities students and trying to establish relations with student...

02 May 2020 7,097 3 View

How to improve the thorn resistance of textiles?

Unlike body armor, the body armor faces a smaller area of knife tip. Although the energy is small, some body armor does not prevent the knife from penetrating.Moreover, if the use of composite...

24 November 2019 928 4 View

Theoretical max-min of Gibbs free energy?

Hello all, Does anyone know if there is a theoretical maximum and minimum for the Gibbs free energy? In other words, under constant physiological conditions, can one calculate the possible...

04 July 2019 8,773 5 View

Optical and radiative properties of molten salt?

The thermal radiation transfer in molten salt is usually neglected in researches. Does anyone knows the reason ? Are there any research (experimental data) focusing on the absorption coefficient...

30 June 2019 1,243 3 View

Can anyone help me convert the concentration of human anti-hepatitis B antibody (Hepabig) from IU/ml to mg/ml?

I am using Hepabig brand which only states 100 IU/0.5 ml concentration of antibody but no mg/ml immunoglobulin concentration estimate.

05 March 2019 5,808 15 View

What about Multiple comparisons in a linear mixed model in R?

I'm doing the analysis with a linear mixed model.Because the sample size is different, the Scheffe test is used for the multiple comparison of my data. However, I do not know how to write code in...

09 January 2019 2,517 6 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Books on Monte Carlo methods?

Hi everyone. I took a basic course on Markov Chains, and know a little about Monte Carlo Stimulations and Methods. But I never got to the part of spreadsheets. If anyone can help direct me to a...

13 February 2021 5,261 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Are there any compounds that can increase the viability and stimulate cell viability and cell division?

I have trouble growing a cell line that I had just purchased from a cell line distributor. The cells I bought were produced in 2016 and when they were seedling they couldn't grow. There were some...

02 February 2021 3,751 5 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Buffer for protein solubilization after acetone/AS precipitation that is compatible with cell stimulation?

Hello! I am precipitating some proteins (with either acetone or ammonium sulphate) and I need to resuspend the protein pellet in a buffer that properly keeps the proteins soluble AND which is...

27 January 2021 6,958 4 View