Please advise! What is missing in my Immunoprecipitation protocol? how to avoid antibody contamination in my elute?

Hi I am working on immunoprecipitating AMPK alpha protein from my lysates. I am using a sigma aldrich IP kit that includes beads mixed A+G proteins. I tried 3 different elution techniques:...

29 January 2020 4,416 7 View

How can we transfer data from MATLAB to OPNET how the interface will be?

good evening, i work on IEEE 14 bus system on MATLAB and i need to transfer the data to OPNET 14.5 . the communication between these two softwares seems complicated and i didn't find how to do...

25 November 2018 6,642 2 View

Physical properties of Al- 10wt% Mg alloy?

Hello; Please, I neead the physical properties of Al- 10wt% Mg alloy to begin a new reaserch. with my best regards to all

07 August 2018 9,152 1 View

Is it important to change 10 % neutral buffered formalin ?

01 June 2016 8,873 7 View

Are there any problems if i reserved fish tissues in deep freezer at -20 for later histopathology?

02 May 2016 981 8 View

WURSS-11 - how to apply in research practice?

Hello, in the framework of my master's thesis, I would like to assess the efficacy of probiotic on upper respiratory tract infection (URTI) incidence and symptom ratings in athletes under...

07 January 2021 2,284 1 View

Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Is there any highthrough put method for screening of microbes having Bsh activity ? #probiotics ?

can anybody help me with In-vivo screening strategy for microbes having Bsh activity? I want to explore them for cholesterol lowering activity?

11 October 2020 6,355 3 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

Apart from using probiotics, following biosecurity and good management practices, is there any cure/medicine for White feces disease in Vannamei now?

the gut related diseases like White feaces, white muscle, white gut are doing much damage to shrimp by retarding growth and increasing FCR and eventually heavy losses. is there any medicine or...

07 October 2020 5,213 3 View

Can anyone suggest which cell lines should be used to study probiotic adhesion study?

For probiotic adhesion study, many of the researchers used HT29 and Caco2 cell lines, among that which will be more preferred one

27 September 2020 968 4 View

Can anyone advise me in selecting a good pair of universal primer/primers for identification of lactic acid bacteria (probiotics)?

when I blast the existed primers specially 16s rDNAs , I cant get good results.

21 September 2020 1,584 4 View

Which animal model is better suited for hypercholesterolemia and probiotic studies?

We are planning an in vivo study on the relationship between hypercholesterolemia and probiotics. Unfortunately, we don't have the chance to work with hamsters, we have very few options for...

30 August 2020 8,794 6 View

How do other researchers measure relative quality control in production of ancient Glass?

I find it interesting that there is very little emphasis on uniformity of production when it comes to early glass manufacture. To me, it is an essential ingredient when analysing the differences...

02 June 2020 2,724 1 View

How to make probiotic in different concentration?

I want to know how to make probiotic in different concentration. I am planning to probiotic experiment with different concentration. I also want to know how to make the freeze dry probiotic powder...

14 April 2020 1,197 2 View