We are trying to prepare a large amount of purified rosmarinic acid for testing its biological activities and medicinal products. Rosmarinic acid is well known for its antioxidant, anti-inflammation, anti-bacterial effects, etc.
Dear Sir,
Above this very useful suggestion and valuable for isolation of Rosmarinic acid from different source. I personally think that from leave firstly you could try with Sephadex LH-20 column then you can go with gravity (long column) prep HPLC (C18, 5micron particle size and 0.1% formic acid in aqueous phase and methanol with variable flow rate and develop a suitable method.
Preparative separation and purification of rosmarinic acid from perilla seed meal via combined column chromatography
Tang, Weizhuo; Sun, Baoshan; Zhao, Yuqing Journal Journal of Chromatography B , Volume 947 – Feb 1, 2014
Hope this is useful
You first go for simple hydrodistillation method by using clavenger assembly. Then you check the content of acid you want by simple chromatographic method in that. The partial purified fraction may you get here.
The original isolation of rosmarinic acid by Maria Scarpatti of the University of Rome (in the 1950's) was from Rosmarinus officinalis was published. A tea from rosemary leaves was treated with lead acetate (sugar of lead) solution to cause the precipitation of most of the catechols present. The filtered solids were resuspended in water and hydrogen sulfide was bubbled in until the PbS precipitation was complete. The PbS was removed by vacuum filtration and the clarified aqueous phase was extracted in a separatory funnel with DIETHYL ETHER to give on concentration rather pure rosmarinic acid. Subsequent extraction of the parent liquor with ethyl acetate brought out another fraction of (presumably) catechol acids. The use of ether for extraction was the key to separation of the rosmarinic acid in a crystallizable form from other plant components. I did this experiment with my own hands in 1974 to obtain the pure rosmarinic acid we published the 13C specturm of: Kelley, Charles J.; Harruff, Richard C.; Carmack, Marvin. Polyphenolic acids of Lithospermum ruderale. II. Carbon-13 nuclear magnetic resonance of lithospermic and rosmarinic acids. Journal of Organic Chemistry (1976), 41(3), 449-55.
Thanks all those who answer my questions.Some can be done here in our University , but some cannot be done.
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