Glycation is defined as non-enzymatic glycosylation resulting by chemical interaction between a protein or lipid molecule with a sugar molecule. We have found that glycation inhibition activity is directly related to the content of polyphenolic compounds in plants.
Polyphenols may directly react with BSA preventing the glycosylation.
Dr Suttajit -
Greetings!
This may be related the antioxidant property of phenolics and flavonoids. In certain studies, plant the extracts with glycation inhibitory activity also exhibited showed antioxidative activity. Protein glycation inhibitory activity may be owing to the reducing and hydrogen donating (free radical scavenging) activity of the molecules.
Your suggestion is also possible as flavonoids bind to albumin.
Dear All,
Phytochemicals interact with the residues in the bovine serum albumin especially arginine which prevents the exposure of the residues to glucose. This will reflect the anti-glycation potential.
Thank you, Govindarajan! The interaction potential of dietary flavonoids (flavonols such as quercetin) is of an intermediary range. Cheers...
Dear Dr Suttajit and Govindarajan:
Wu CH, Yen GC. Inhibitory effect of naturally occurring flavonoids on the formation of advanced glycation endproducts. J Agric Food Chem. 2005 20;53:3167-73. The results indicated that protein glycation was accompanied by oxidative reactions, as the ESR spectra showed a clear-cut radical signal. Statistical analysis showed that inhibitory capability of flavonoids against protein glycation was remarkably related to the scavenging free radicals derived from glycoxidation process. Consequently, the inhibitory mechanism of flavonoids against glycation was, at least partly, due to their antioxidant properties.
Other sites of polyphenol/flavonoid action: it is well known that glycation induces covalent modification of proteins by the introduction of carbonyl groups into amino acid residues of proteins. Berlett BS, Stadtman ER. Protein oxidation in aging, disease, and oxidative stress. J Biol Chem. 1997 Aug 15;272:20313-6. Glycation diminishes the content of free thiol groups in BSA.
Bhattacherjee A, Chakraborti AS. Inhibitory effect of Piper betle Linn. leaf
extract on protein glycation--quantification and characterization of the
antiglycation components. Indian J Biochem Biophys. 2013, 50:529-36. Fraction A may exert antiglycation activity through the antioxidant properties of its phenolic compounds, particularly rutin, a quercetin glycoside.
Regards…
Thank you , Dr. Chithan C Kandaswami . It is good to know you from the SUNYAB where I was studying for my Ph.D. under the supervision of Prof. Richard J. Winzler (1967-1971).
Thank you, kindly Dr. Maitree Suttajit. It is great. As you are aware of, SUNYAB has immensely expanded over the years, and the 2,500 acre (plus another 2,000 potentially available at the James Audubon Park) Amherst campus (once isolated) has also become lively; health sciences campus in downtown Buffalo is being completed and the Buffalo biomedical campus nearby make this a huge biomedical center.
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