Is there anyone who can provide me Agrobacterium tumefaciens strain LBA 4404 or any company that sells the strain?

More Monoj Sutradhar's questions See All

What is the Z-interval in the confocal laser scanning microscopy analysis? How to get it? shall i fix it before doing scanning?

I am doing CLSM (confocal laser scanning microscopy) of nafion film. I am doing z-scanning. But i am having problem in setting the Z-interval.

27 March 2024 7,852 1 View

What does this air pressure scale means when i increase or decrease the air flow?

I am studying fluorescence Spectroscopy with relative humidity. I am creating the humidity passing air..but, i want to know what does this air pressure scale means?

27 December 2023 1,632 0 View

I wanted to study swelling of nafion thin film using 500 ul liquidCell alpha ellipsometry. How do i approach and is there any relevant paper on that?

I measured the Thickness of the thin film using ellipsometry. But, now i want to study the swelling of thin film using 500 ul liquidCell alpha ellipsometry. Looking for some reference papers and...

19 December 2023 9,193 5 View

What does that marked air pressure scale means when i increase or decrease the air flow?

I am using this Fluorescence Spectroscopy from Horiba Pti machine. I run this whith Creating humidity inside the chamber of machine. So, when i create the air flow, the air pressure scale...

15 December 2023 8,355 0 View

How to prevent condensation inside a chamber where i am increasing humidity in the chamber slowly?

I am doing a fluorescence spectroscopy experiment of a nafion thin film with HPTS dye and running it using salt solutions for humidity. But, at High Humidity such as 70% it starts condensation...

25 November 2023 2,987 4 View

How to prepare of Warfarin Solution?

I want to prepare a warfarin solution from warfarin tablets. So, can anyone tell me how to prepare a warfarin solution from a warfarin tablet and what will be the composition of the warfarin tablet?

04 August 2023 5,223 1 View

Which properties of nanocatalysts form strong chemical bonds that create oxidation?

Example, Pt and other nanocatalysts with CeO2 or other materials oxidize.

18 April 2023 5,635 2 View

Which python library is used for computing dft( density functional theory ) as siesta python interface?

Picture if possible

14 April 2023 7,460 2 View

How to describe π-π interaction?

Without getting a single crystal of the compound, how can we explain the π-π interaction of the compound? Is there any characterization method available?

30 March 2023 9,807 0 View

What are the possible physicochemical treatments for the surface modification of microalgal cell ?

Looking for the efficient type of physicochemical treatment for the surface modification of microalgal cells and its application in heavy metals remediation.

07 September 2022 3,088 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Yuan-Yeu Yau

Company ThermoFisher sells this strain if you cannot obtain one from other labs. See here:

ElectroMAX™ A. tumefaciens LBA4404 Cells

https://www.thermofisher.com/order/catalog/product/18313015

Surender Khatodia

Look in MTCC, IMTECH, Chandigarh or you can ask anybody in NRCPB, New Delhi. Let me know if you need some ones contact.

ElectroMAX™ A. tumefaciens LBA4404 Cells mentioned above is an electroporation-grade LBA4404 competent cells. After you buy it, you can use it to make your own competent LBA4404 in the lab afterwards. There are plenty protocols for making competent Agrobacterium cells online.

Manoj Kumar

Yes I can