How do you choose the detergent concentration for protein purification?

hey i am using 2%OG and 1%DDM what concentration do i need to use with DM? is there a formula to calculate? Why usualy using 1%DDM and 2% OG (what % is higher then the CMC?) thanks

31 December 2018 3,925 8 View

Is using synonyms or antonyms enough in paraphrasing a text? Is doing this considered Paraphrasing?

When coming to write a research paper or a report, most EFL students tend to paraphrase through using synonyms and antonyms. Thinking that it is enough. How can we deal with such a thing?

26 December 2018 5,894 15 View

How to calculate the sample size? Can I cite the same article(s) in more than one research?

I'm planning to do a research based on questionnaire/ survey and population are clinical researchers from the whole country. The count of researchers in the country in unknown (I don't know how...

07 March 2018 2,405 4 View

In survey, not all questions answered by respondents. What should I do ?

for example, total respondents are 100. 50 answered Q6, 45 answered Q7 , 60 answered Q8. What should I do to write the results to be acceptable for the journal reviewers? The survey has...

04 February 2018 1,164 4 View

When dissolving expanded polystyrene (EPS) in acetone what fumes are produced? And is there a way of measuring this?

I am researching and developing alternate methods of recycling EPS and am getting interesting results via dissolution in acetone. However I want to make an evaluation of the potentially hazardous...

15 January 2018 478 2 View

How do calculate decolorizing activity of bacteria by using uv-vis spectrophotometer?

27 June 2017 596 3 View

How can i produce genomic analysis (DNA extract) with very low concentration of DNA from Yeast?

hey all i need to extract DNA from yeast cells. but how can i do it from one yeast or very low number of yeast cells. is there a special protocol for it? Thanks May

17 June 2017 5,842 9 View

Does exist a gene expression programming toolbox for matlab?

20 May 2017 3,852 4 View

For recharging Hitrap Talon 1ml cobalt, can I use Cl2Co*6H2O?

The GE official protocol says I need 50mM Cl2Co, so should I calculate only by the Cl2Co MW? Thank you!

30 April 2017 3,961 2 View

What settings should i be using on Primer3 and Primer BLAST to prevent primers with potential off-target products from showing up in my results?

22 March 2017 6,290 3 View

What is corrct way to define ports when simulating FSS and surface waves in CST?

I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...

19 January 2021 6,590 4 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Hi Is there any one, who help me in processing my raw data files of some mushrooms of LC-MS , by using MZmine2, MS- Dial etc ?

I have received some of my LC-MS raw files but I can not process it by using MZmine2. MS Dial, for compounds detection. Thanks in advance.

02 November 2020 3,564 5 View

No title

mushrooms play a vital role in human life used for food, medicine, and chemical industries, many edible mushroom-like morels can not be grown by a human, and this type of mushrooms are called...

18 October 2020 1,457 12 View

What are the equation and conversion factor to estimate protein content from mushroom?

Total nitrogen was calculated with Kjeldahl method, I found different equations and factors ''Total nitrogen content of shrimp waste was determined using the Kjeldahl method. Separately, pure...

17 August 2020 4,572 9 View

Does it matter what order of extraction solvent is used for extracting bioactive compounds from medicinal mushrooms (elaborate description below)?

Hi, My question is regarding medicinal mushroom extraction, but may also apply to broader extraction knowledge of all biota. I understand, that water and ethanol as extraction solvents (along...

25 June 2020 1,113 9 View

How to work with diversity indexes data while working with aleatory transects inside an area?

Hello! I've used the aleatory transect methodology to survey for mushrooms in six different areas. We've done 4 samples (transects) in which area along 4 months , one per month. I've already...

07 April 2020 9,664 3 View

I would like to know what is the standard method of cultivation and propagation of truffle mushrooms in the laboratory and field?

I would like to know what is the standard method of cultivation and propagation of truffle mushrooms ( Tuber aestivum , Tuber macrosporum , Tuber brumale ) in the laboratory and field? There...

11 December 2019 1,288 3 View

Hi, i am confused with 'hot water extraction' and 'maceration extraction'. in my point of view both are same, please share your views?

hot water extraction of mushroom polysaccharides, Maceration extraction of bioactive compounds from mushrooms

10 December 2019 4,789 3 View

I would like to know what is the standard method of cultivation and propagation of truffle mushrooms in the laboratory and field?

I would like to know what is the standard method of cultivation and propagation of truffle mushrooms ( Tuber aestivum , Tuber macrosporum , Tuber brumale ) in the laboratory and field? There...

30 November 2019 6,662 3 View