What is the best ITS primers for discrimination of fungi species?

Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by a few limitations, the ITS will remain as the key choice for fungal identification. I usually see the ITS 1F...

11 August 2020 3,597 10 View

How can I prepare Lysozyme digestion buffer for Invitrogen DNA extraction kit (Gram positive bacteria)?

I'd like to prepare a Lysozyme and Lysozyme Digestion Buffer (25mM Tris-HCL, pH 8.0, 2.5mM EDTA, 1% Triton X-100) for bacterial cell lysate with a final Lysozyme concentration of 20mg/mL. I need...

29 July 2020 2,818 2 View

How bin size affects Particle Size Distribution?

Hi, I have question which I could not find an appropriate answer. By default, each laser diffraction instrument plots PSD using different bin sizes on the x-axis. This means, using default...

21 October 2019 6,899 3 View

Any guidelines for number of cores/problem size in parallel CFD simulations?

Hello, I constantly submit parallel jobs in a shared HPC cluster for my OpenFOAM flow simulations and like everyone else, I have to decide how many cores I need. Our cluster runs constantly over...

11 May 2019 8,329 6 View

Simulation and measurement results are different. What can be the reason?

I simulated a patch antenna. I saw that the best S11 value is at 5.9GHz but when I measured I saw that it works at 5.93 GHz. What can be the reason?

23 September 2017 9,104 2 View

How can I use PCR without genomic extraction on N2a cells?

I have primers and i think i have to lysis the N2a cells but after that i couldn't find any protocol like how much i must add for template or other things.

06 June 2017 8,442 3 View

How can we draw or get the centerline of a surface using Solidworks?

I would like to learn drawing or getting centerline of a surface in Solidworks. I search on internet but I found limited information. I found a programs like VMTKLab.There is a section for...

12 May 2015 2,748 7 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View