How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View

What kind of dialysis cassette is needed for CaCl2 in silk fibroin dissolving?

Hi! Recently, I am working on silk fibroin extraction for biomedical application. I have some problem in details of extraction and dissolving of silk fibroin. For example, I don't know what kind...

26 June 2020 3,927 1 View

What kind of dialyses cassette is require for extraction of silk fibroin base on nature protocol 2011 (Attached in following)?

I have some question about the laboratory process of extraction silk fibroin from Bombyx mori base the Prof. Kaplan protocol. How much Silk cocoon need for 20 experiments? How dialyzes the final...

15 June 2020 8,694 1 View

Is there a spider silk stain (dye)? I need a stain to identify spider silk or webbing from other material?

I need to try to identify spider silk from other materials (soil, insect carcass, plant materials) and would like to find a stain or dye that would help visualize the spider silk. If it could be...

08 September 2019 2,174 5 View

How can I find a copy of the Job Demands and Job Resources Measure (Survey)?

I'm looking for a copy of the Job Demands and Job Resources Measure to use for my dissertation study. This is a 20-item survey that was used in the following study: Fodor, Daniel P., Antoni,...

07 August 2019 2,744 3 View

Dye extraction from silk fabric?

Hello. I work in a mass spec lab. I have different types of antic silk fabric, dyed with various unknown pigments. How can I extract the pigment to undergo mass spec analysis (MALDI-TOF)?

04 May 2019 9,523 1 View

To what extent have sustainability criteria been included in this project?

In its definition of the new Silk Road, China is keen to present its own development experience in the context of its introduction to this project as one of the models that can be utilized, while...

04 May 2019 3,620 1 View

Any tallent young is willing to China?

31 December 2018 5,858 1 View

Can anyone suggest me the best way to remove entrapped formic acid from silk fibroin hard gel? And how to quantify it?

Can anyone suggest me the best way to remove entrapped formic acid from silk fibroin hard gel? And how to quantify it?

09 October 2018 3,762 0 View