Global management market of courses & marketing to improve quality in Pt Treatment or science or mainly Bureaucracy?

12 October 2020 9,272 1 View

SEM: What does CFI = 1 & RMSEA = 0 indicate in over-identified models?

I'm suspicious about "perfect fit" in my SEM path analysis, with fit indices of CFI = 1 and RMSEA = 0 which I would expect from an just-identified model. However, since I have 3 degrees of...

08 August 2020 1,492 4 View

Data on most frequently researched KSAOs or personality traits?

I'm looking for meta analyses or reviews, summarizing which knowledge, skills, abilities and other characteristics (KSAOs, especially personality traits) are most frequently researched in...

22 May 2020 332 1 View

AC/DC inverters: Are there any standards or laws regulation DC output on the AC Grid?

Hi, we are working on internal specifications on On-Board Charger for Battery electric vehicles. We have seen some field failures on older generations. We are suspecting that e.g. PV AC/DC...

15 December 2019 6,887 1 View

How to test unidimensionality for polytomous data with IRT-models in R?

I would be thankful if someone could give me a short breakdown on how to test unidimensionality of polytomous data (likert-scale) using IRT-models in R. As it is my understanding that the...

22 November 2019 6,730 7 View

How do you optimize your research design for questionnaires with large item pools?

While developing a questionnaire to measure several personality traits in a somewhat unconventional way, I now seem to be facing a dilemma due to the size of my item pool. The questionnaire...

30 May 2019 2,633 4 View

What factors generally improve inter-rater reliability?

I'm developing a questionnaire to assess observational data in an area of research which is notoriously prone to low inter-rater reliability. Hence, I'm looking for factors in questionnaire design...

03 December 2018 4,006 3 View

Machine learning in economic research?

What is your take on machine learning as an applied method in economic research. is it a game changer or will it not impact your approach to research?

22 July 2018 7,401 5 View

What is the average magnitude of correlations between common personnel selection methods?

Looking for recent (preferably meta-analytic) findings that yielded estimates of the proportion of shared variance among common personnel selection methods such as structured and unstructured...

11 December 2017 1,946 3 View

Does anyone have information about the Ecology of the Chrysomelid beetle "Chrysolina cerealis"?

We have to manage one its the last populations in Northern Germany and would be glad to gather knowledge!

07 November 2016 1,225 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View